Abstract
A modified suppression subtractive hybridization assay was performed to uncover genes induced by all-trans retinoic acid in cultured smooth muscle cells (SMC). Northern blotting studies confirmed the induction of 14 genes, many of which have heretofore been unrecognized as retinoid-inducible. Temporal expression and cycloheximide studies allowed us to categorize these genes as either immediate-early (LOX-1, endolyn, Stoned B/TFIIAα/β-like factor, Src Suppressed C Kinase Substrate, and tissue transglutaminase) or delayed (cathepsin-L, ceruloplasmin, epithelin, importin α, α8-integrin, lactate dehydrogenase B, retinol dehydrogenase, spermidine/spermine N1-acetyltransferase, and VCAM-1) retinoid-response genes. A survey of rat tissues showed two of the genes (tissue transglutaminase and α8-integrin) to be highly restricted to vascular tissue. In situ hybridization verified expression of both tissue transglutaminase and α8-integrin to SMC in balloon-injured rat carotid artery. These findings unveil a new retinoid-response gene set that should be exploited to define molecular pathways involved in the antagonistic effects of retinoids on SMC growth and neointimal formation.
Original language | English (US) |
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Pages (from-to) | 475-482 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 281 |
Issue number | 2 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Cloning
- Differentiation
- Growth
- Retinoid
- Subtractive hybridization
- Tretinoin
- Vascular
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology