A novel retinoid-response gene set in vascular smooth muscle cells

J. Chen, K. M. Maltby, J. M. Miano

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A modified suppression subtractive hybridization assay was performed to uncover genes induced by all-trans retinoic acid in cultured smooth muscle cells (SMC). Northern blotting studies confirmed the induction of 14 genes, many of which have heretofore been unrecognized as retinoid-inducible. Temporal expression and cycloheximide studies allowed us to categorize these genes as either immediate-early (LOX-1, endolyn, Stoned B/TFIIAα/β-like factor, Src Suppressed C Kinase Substrate, and tissue transglutaminase) or delayed (cathepsin-L, ceruloplasmin, epithelin, importin α, α8-integrin, lactate dehydrogenase B, retinol dehydrogenase, spermidine/spermine N1-acetyltransferase, and VCAM-1) retinoid-response genes. A survey of rat tissues showed two of the genes (tissue transglutaminase and α8-integrin) to be highly restricted to vascular tissue. In situ hybridization verified expression of both tissue transglutaminase and α8-integrin to SMC in balloon-injured rat carotid artery. These findings unveil a new retinoid-response gene set that should be exploited to define molecular pathways involved in the antagonistic effects of retinoids on SMC growth and neointimal formation.

Original languageEnglish (US)
Pages (from-to)475-482
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume281
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • Cloning
  • Differentiation
  • Growth
  • Retinoid
  • Subtractive hybridization
  • Tretinoin
  • Vascular

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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