A Novel RNA In Situ Hybridization Assay for the Long Noncoding RNA SChLAP1 Predicts Poor Clinical Outcome After Radical Prostatectomy in Clinically Localized Prostate Cancer

Rohit Mehra, Yang Shi, Aaron M. Udager, John R. Prensner, Anirban Sahu, Matthew K. Iyer, Javed Siddiqui, Xuhong Cao, John Wei, Hui Jiang, Felix Y. Feng, Arul M. Chinnaiyan

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Long noncoding RNAs (lncRNAs) are an emerging class of oncogenic molecules implicated in a diverse range of human malignancies. We recently identified SChLAP1 as a novel lncRNA that demonstrates outlier expression in a subset of prostate cancers, promotes tumor cell invasion and metastasis, and associates with lethal disease. Based on these findings, we sought to develop an RNA in situ hybridization (ISH) assay for SChLAP1 to 1) investigate the spectrum of SChLAP1 expression from benign prostatic tissue to metastatic castration-resistant prostate cancer and 2) to determine whether SChLAP1 expression by ISH is associated with outcome after radical prostatectomy in patients with clinically localized disease. The results from our current study demonstrate that SChLAP1 expression increases with prostate cancer progression, and high SChLAP1 expression by ISH is associated with poor outcome after radical prostatectomy in patients with clinically localized prostate cancer by both univariate (hazard ratio = 2.343, P =.005) and multivariate (hazard ratio = 1.99, P =.032) Cox regression analyses. This study highlights a potential clinical utility for SChLAP1 ISH as a novel tissue-based biomarker assay for outcome prognostication after radical prostatectomy.

Original languageEnglish (US)
Pages (from-to)1121-1127
Number of pages7
JournalNeoplasia (United States)
Volume16
Issue number12
DOIs
StatePublished - Dec 2014
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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    Mehra, R., Shi, Y., Udager, A. M., Prensner, J. R., Sahu, A., Iyer, M. K., Siddiqui, J., Cao, X., Wei, J., Jiang, H., Feng, F. Y., & Chinnaiyan, A. M. (2014). A Novel RNA In Situ Hybridization Assay for the Long Noncoding RNA SChLAP1 Predicts Poor Clinical Outcome After Radical Prostatectomy in Clinically Localized Prostate Cancer. Neoplasia (United States), 16(12), 1121-1127. https://doi.org/10.1016/j.neo.2014.11.006