A reverse nuclear factor-κB element in the rat type II nitric oxide synthase promoter mediates the induction by interleukin-1β and interferon-γ in rat aortic smooth muscle cells

Xingwu Teng, Hanfang Zhang, Connie Snead, John D. Catravas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The rat type II nitric oxide synthase (iNOS) promoter contains two nuclear factor-κB (NF-κB)-binding sites, one upstream (-965 to -956 bp) and one downstream (-107 to -98 bp), which are important for iNOS induction. We have identified a third NF-κB site located at -901 to -892 bp whose sequence is identical to that of the upstream site but with the opposite orientation ('the reverse NF-κB site'). We hypothesized that the reverse NF-κB site, like the other two sites, is important for iNOS induction. With the use of a rat iNOS promoter fragment of -906 to -887 bp as probe, electrophoretic mobility shift assays were performed on nuclear proteins extracted from rat aortic smooth muscle cells (RASMCs) treated with interleukin-1β (IL-1β, 100 U/ml) ± interferon-γ (IFN-γ, 250 U/ml) for 30 min. IL-1β, but not IFN-γ, induced a DNA-protein complex that was supershifted by either anti-NF-κB p50 or anti-NF-κB p65 antibody. The functionality of the reverse NF-κB site was evaluated by mutation experiments and transfection assays. The wild-type and mutated -1.4 kb rat iNOS promoter-luciferase constructs were transfected into RASMCs. Compared with the wild type, reverse-NF-κB site (-901 to -892 bp) deletion, substitution of T for C at -894 bp, and substitution TTT for CCC at -896 to -894 bp decreased the IL-1β-induced promoter activity by 67% (p < 0.001), 45% (p < 0.001), and 56% (p < 0.001), respectively. These deletion/substitutions also decreased the IL-1β- and IFN-γ-induced promoter activity by 74% (p < 0.001), 53%(p < 0.001), and 63% (p < 0.001), respectively. In conclusion, a p50 and p65 NF-κB heterodimer binds to a reverse-NF-κB site on the rat iNOS promoter and contributes to iNOS induction by IL-1β and IFN-γ in RASMCs. (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalGeneral Pharmacology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 2000

Fingerprint

Nitric Oxide Synthase Type II
Interleukin-1
Interferons
Smooth Muscle Myocytes
Electrophoretic Mobility Shift Assay
Nuclear Proteins
Luciferases
Transfection
Binding Sites
Mutation
Antibodies
DNA
Proteins

Keywords

  • IFN-γ
  • IL-1β
  • iNOS promoter
  • NF-κB
  • Rat
  • Vascular smooth muscle cells

ASJC Scopus subject areas

  • Pharmacology

Cite this

A reverse nuclear factor-κB element in the rat type II nitric oxide synthase promoter mediates the induction by interleukin-1β and interferon-γ in rat aortic smooth muscle cells. / Teng, Xingwu; Zhang, Hanfang; Snead, Connie; Catravas, John D.

In: General Pharmacology, Vol. 34, No. 1, 01.01.2000, p. 9-16.

Research output: Contribution to journalArticle

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abstract = "The rat type II nitric oxide synthase (iNOS) promoter contains two nuclear factor-κB (NF-κB)-binding sites, one upstream (-965 to -956 bp) and one downstream (-107 to -98 bp), which are important for iNOS induction. We have identified a third NF-κB site located at -901 to -892 bp whose sequence is identical to that of the upstream site but with the opposite orientation ('the reverse NF-κB site'). We hypothesized that the reverse NF-κB site, like the other two sites, is important for iNOS induction. With the use of a rat iNOS promoter fragment of -906 to -887 bp as probe, electrophoretic mobility shift assays were performed on nuclear proteins extracted from rat aortic smooth muscle cells (RASMCs) treated with interleukin-1β (IL-1β, 100 U/ml) ± interferon-γ (IFN-γ, 250 U/ml) for 30 min. IL-1β, but not IFN-γ, induced a DNA-protein complex that was supershifted by either anti-NF-κB p50 or anti-NF-κB p65 antibody. The functionality of the reverse NF-κB site was evaluated by mutation experiments and transfection assays. The wild-type and mutated -1.4 kb rat iNOS promoter-luciferase constructs were transfected into RASMCs. Compared with the wild type, reverse-NF-κB site (-901 to -892 bp) deletion, substitution of T for C at -894 bp, and substitution TTT for CCC at -896 to -894 bp decreased the IL-1β-induced promoter activity by 67{\%} (p < 0.001), 45{\%} (p < 0.001), and 56{\%} (p < 0.001), respectively. These deletion/substitutions also decreased the IL-1β- and IFN-γ-induced promoter activity by 74{\%} (p < 0.001), 53{\%}(p < 0.001), and 63{\%} (p < 0.001), respectively. In conclusion, a p50 and p65 NF-κB heterodimer binds to a reverse-NF-κB site on the rat iNOS promoter and contributes to iNOS induction by IL-1β and IFN-γ in RASMCs. (C) 2000 Elsevier Science Inc.",
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AU - Catravas, John D.

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N2 - The rat type II nitric oxide synthase (iNOS) promoter contains two nuclear factor-κB (NF-κB)-binding sites, one upstream (-965 to -956 bp) and one downstream (-107 to -98 bp), which are important for iNOS induction. We have identified a third NF-κB site located at -901 to -892 bp whose sequence is identical to that of the upstream site but with the opposite orientation ('the reverse NF-κB site'). We hypothesized that the reverse NF-κB site, like the other two sites, is important for iNOS induction. With the use of a rat iNOS promoter fragment of -906 to -887 bp as probe, electrophoretic mobility shift assays were performed on nuclear proteins extracted from rat aortic smooth muscle cells (RASMCs) treated with interleukin-1β (IL-1β, 100 U/ml) ± interferon-γ (IFN-γ, 250 U/ml) for 30 min. IL-1β, but not IFN-γ, induced a DNA-protein complex that was supershifted by either anti-NF-κB p50 or anti-NF-κB p65 antibody. The functionality of the reverse NF-κB site was evaluated by mutation experiments and transfection assays. The wild-type and mutated -1.4 kb rat iNOS promoter-luciferase constructs were transfected into RASMCs. Compared with the wild type, reverse-NF-κB site (-901 to -892 bp) deletion, substitution of T for C at -894 bp, and substitution TTT for CCC at -896 to -894 bp decreased the IL-1β-induced promoter activity by 67% (p < 0.001), 45% (p < 0.001), and 56% (p < 0.001), respectively. These deletion/substitutions also decreased the IL-1β- and IFN-γ-induced promoter activity by 74% (p < 0.001), 53%(p < 0.001), and 63% (p < 0.001), respectively. In conclusion, a p50 and p65 NF-κB heterodimer binds to a reverse-NF-κB site on the rat iNOS promoter and contributes to iNOS induction by IL-1β and IFN-γ in RASMCs. (C) 2000 Elsevier Science Inc.

AB - The rat type II nitric oxide synthase (iNOS) promoter contains two nuclear factor-κB (NF-κB)-binding sites, one upstream (-965 to -956 bp) and one downstream (-107 to -98 bp), which are important for iNOS induction. We have identified a third NF-κB site located at -901 to -892 bp whose sequence is identical to that of the upstream site but with the opposite orientation ('the reverse NF-κB site'). We hypothesized that the reverse NF-κB site, like the other two sites, is important for iNOS induction. With the use of a rat iNOS promoter fragment of -906 to -887 bp as probe, electrophoretic mobility shift assays were performed on nuclear proteins extracted from rat aortic smooth muscle cells (RASMCs) treated with interleukin-1β (IL-1β, 100 U/ml) ± interferon-γ (IFN-γ, 250 U/ml) for 30 min. IL-1β, but not IFN-γ, induced a DNA-protein complex that was supershifted by either anti-NF-κB p50 or anti-NF-κB p65 antibody. The functionality of the reverse NF-κB site was evaluated by mutation experiments and transfection assays. The wild-type and mutated -1.4 kb rat iNOS promoter-luciferase constructs were transfected into RASMCs. Compared with the wild type, reverse-NF-κB site (-901 to -892 bp) deletion, substitution of T for C at -894 bp, and substitution TTT for CCC at -896 to -894 bp decreased the IL-1β-induced promoter activity by 67% (p < 0.001), 45% (p < 0.001), and 56% (p < 0.001), respectively. These deletion/substitutions also decreased the IL-1β- and IFN-γ-induced promoter activity by 74% (p < 0.001), 53%(p < 0.001), and 63% (p < 0.001), respectively. In conclusion, a p50 and p65 NF-κB heterodimer binds to a reverse-NF-κB site on the rat iNOS promoter and contributes to iNOS induction by IL-1β and IFN-γ in RASMCs. (C) 2000 Elsevier Science Inc.

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