A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1

Vaibhav Tiwari, Christian Clement, Michael B Duncan, Jinghua Chen, Jian Liu, Deepak Shukla

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Membrane fusion induced by herpes simplex virus (HSV) is required for both entry and cell-to-cell spread. It is mediated by the viral glycoprotein gB, gD, gH-gL and gD receptors. Although 3-O-sulfated heparan sulfate (3-OS HS) is a receptor for HSV-1 entry, the requirement for heparan sulfate in the fusion process has been ruled out. Here, it is demonstrated that cells expressing 3-OS HS, generated by D-glucosaminyl 3-O-sulfotransferase isoforms-3 and/or -5 (3-OST-3 and 3-OST-5), fused with cells expressing the four glycoproteins. The cell fusion observed exhibited similar requirements but was independent of protein receptors, HVEM or nectin-1. Additionally, removal of 3-OS HS from the cell surface by heparinase-I treatment and, in separate experiments, the presence of soluble 3-OST-3- and 3-OST-5-modified HS, significantly inhibited fusion. Taken together, these results indicate that 3-OS HS can play a crucial role in virus entry and cell fusion.

Original languageEnglish (US)
Pages (from-to)805-809
Number of pages5
JournalJournal of General Virology
Volume85
Issue number4
DOIs
StatePublished - Apr 1 2004

Fingerprint

Heparitin Sulfate
Cell Fusion
Human Herpesvirus 1
Virus Internalization
Simplexvirus
Receptors, Tumor Necrosis Factor, Member 14
Glycoproteins
Heparin Lyase
Membrane Fusion
Protein Isoforms

ASJC Scopus subject areas

  • Virology

Cite this

A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1. / Tiwari, Vaibhav; Clement, Christian; Duncan, Michael B; Chen, Jinghua; Liu, Jian; Shukla, Deepak.

In: Journal of General Virology, Vol. 85, No. 4, 01.04.2004, p. 805-809.

Research output: Contribution to journalArticle

Tiwari, Vaibhav ; Clement, Christian ; Duncan, Michael B ; Chen, Jinghua ; Liu, Jian ; Shukla, Deepak. / A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1. In: Journal of General Virology. 2004 ; Vol. 85, No. 4. pp. 805-809.
@article{c376569526824eb9a7343149a83d6361,
title = "A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1",
abstract = "Membrane fusion induced by herpes simplex virus (HSV) is required for both entry and cell-to-cell spread. It is mediated by the viral glycoprotein gB, gD, gH-gL and gD receptors. Although 3-O-sulfated heparan sulfate (3-OS HS) is a receptor for HSV-1 entry, the requirement for heparan sulfate in the fusion process has been ruled out. Here, it is demonstrated that cells expressing 3-OS HS, generated by D-glucosaminyl 3-O-sulfotransferase isoforms-3 and/or -5 (3-OST-3 and 3-OST-5), fused with cells expressing the four glycoproteins. The cell fusion observed exhibited similar requirements but was independent of protein receptors, HVEM or nectin-1. Additionally, removal of 3-OS HS from the cell surface by heparinase-I treatment and, in separate experiments, the presence of soluble 3-OST-3- and 3-OST-5-modified HS, significantly inhibited fusion. Taken together, these results indicate that 3-OS HS can play a crucial role in virus entry and cell fusion.",
author = "Vaibhav Tiwari and Christian Clement and Duncan, {Michael B} and Jinghua Chen and Jian Liu and Deepak Shukla",
year = "2004",
month = "4",
day = "1",
doi = "10.1099/vir.0.19641-0",
language = "English (US)",
volume = "85",
pages = "805--809",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Society for General Microbiology",
number = "4",

}

TY - JOUR

T1 - A role for 3-O-sulfated heparan sulfate in cell fusion induced by herpes simplex virus type 1

AU - Tiwari, Vaibhav

AU - Clement, Christian

AU - Duncan, Michael B

AU - Chen, Jinghua

AU - Liu, Jian

AU - Shukla, Deepak

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Membrane fusion induced by herpes simplex virus (HSV) is required for both entry and cell-to-cell spread. It is mediated by the viral glycoprotein gB, gD, gH-gL and gD receptors. Although 3-O-sulfated heparan sulfate (3-OS HS) is a receptor for HSV-1 entry, the requirement for heparan sulfate in the fusion process has been ruled out. Here, it is demonstrated that cells expressing 3-OS HS, generated by D-glucosaminyl 3-O-sulfotransferase isoforms-3 and/or -5 (3-OST-3 and 3-OST-5), fused with cells expressing the four glycoproteins. The cell fusion observed exhibited similar requirements but was independent of protein receptors, HVEM or nectin-1. Additionally, removal of 3-OS HS from the cell surface by heparinase-I treatment and, in separate experiments, the presence of soluble 3-OST-3- and 3-OST-5-modified HS, significantly inhibited fusion. Taken together, these results indicate that 3-OS HS can play a crucial role in virus entry and cell fusion.

AB - Membrane fusion induced by herpes simplex virus (HSV) is required for both entry and cell-to-cell spread. It is mediated by the viral glycoprotein gB, gD, gH-gL and gD receptors. Although 3-O-sulfated heparan sulfate (3-OS HS) is a receptor for HSV-1 entry, the requirement for heparan sulfate in the fusion process has been ruled out. Here, it is demonstrated that cells expressing 3-OS HS, generated by D-glucosaminyl 3-O-sulfotransferase isoforms-3 and/or -5 (3-OST-3 and 3-OST-5), fused with cells expressing the four glycoproteins. The cell fusion observed exhibited similar requirements but was independent of protein receptors, HVEM or nectin-1. Additionally, removal of 3-OS HS from the cell surface by heparinase-I treatment and, in separate experiments, the presence of soluble 3-OST-3- and 3-OST-5-modified HS, significantly inhibited fusion. Taken together, these results indicate that 3-OS HS can play a crucial role in virus entry and cell fusion.

UR - http://www.scopus.com/inward/record.url?scp=1842854523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842854523&partnerID=8YFLogxK

U2 - 10.1099/vir.0.19641-0

DO - 10.1099/vir.0.19641-0

M3 - Article

VL - 85

SP - 805

EP - 809

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

IS - 4

ER -