Aberrant bone density in aging mice lacking the adenosine transporter ENT1

David J. Hinton, Meghan Elizabeth McGee Lawrence, Moonnoh R. Lee, Hoi K. Kwong, Jennifer J. Westendorf, Doo Sup Choi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Adenosine is known to regulate bone production and resorption in humans and mice. Type 1 equilibrative nucleoside transporter (ENT1) is responsible for the majority of adenosine transport across the plasma membrane and is ubiquitously expressed in both humans and mice. However, the contribution of ENT1-mediated adenosine levels has not been studied in bone remodeling. With the recent identification of the importance of adenosine signaling in bone homeostasis, it is essential to understand the role of ENT1 to develop novel therapeutic compounds for bone disorders. Here we examined the effect of ENT1 deletion on bone density using X-ray, dual energy X-ray absorptiometry and micro-computerized tomography analysis. Our results show that bone density and bone mineral density is reduced in the lower thoracic and lumbar spine as well as the femur of old ENT1 null mice (>7 months) compared to wild-type littermates. Furthermore, we found increased mRNA expression of tartrate-resistant acid phosphatase (TRAP), an osteoclast marker, in isolated long bones from 10 month old ENT1 null mice compared to wild-type mice. In addition, aged ENT1 null mice displayed severe deficit in motor coordination and locomotor activity, which might be attributed to dysregulated bone density. Overall, our study suggests that ENT1-regulated adenosine signaling plays an essential role in lumbar spine and femur bone density.

Original languageEnglish (US)
Article numbere88818
JournalPLoS One
Volume9
Issue number2
DOIs
StatePublished - Feb 19 2014
Externally publishedYes

Fingerprint

bone density
adenosine
Bone Density
transporters
Bone
Adenosine
Aging of materials
bones
mice
lumbar spine
Bone and Bones
femur
Femur
Equilibrative Nucleoside Transporter 1
Spine
thoracic spine
osteoclasts
dual-energy X-ray absorptiometry
Bone Remodeling
nucleosides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hinton, D. J., McGee Lawrence, M. E., Lee, M. R., Kwong, H. K., Westendorf, J. J., & Choi, D. S. (2014). Aberrant bone density in aging mice lacking the adenosine transporter ENT1. PLoS One, 9(2), [e88818]. https://doi.org/10.1371/journal.pone.0088818

Aberrant bone density in aging mice lacking the adenosine transporter ENT1. / Hinton, David J.; McGee Lawrence, Meghan Elizabeth; Lee, Moonnoh R.; Kwong, Hoi K.; Westendorf, Jennifer J.; Choi, Doo Sup.

In: PLoS One, Vol. 9, No. 2, e88818, 19.02.2014.

Research output: Contribution to journalArticle

Hinton, David J. ; McGee Lawrence, Meghan Elizabeth ; Lee, Moonnoh R. ; Kwong, Hoi K. ; Westendorf, Jennifer J. ; Choi, Doo Sup. / Aberrant bone density in aging mice lacking the adenosine transporter ENT1. In: PLoS One. 2014 ; Vol. 9, No. 2.
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