Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin

E. A. Guzmán, Yen-Hao Chen, J. L. Langowski, A. De Guzman, H. L. Lo, B. Walter, H. K. Muller, A. M. Walker, L. B. Owen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effects of two major forms of prolactin (PRL) were examined on delayed type hypersensitivity (DTH) responses to Candida albicans. Unmodified PRL (U-PRL) had no effect on the DTH response, whereas a molecular mimic of phosphorylated PRL (S179D PRL) significantly inhibited immune responses to this robust antigen. This effect of S179D PRL was not accompanied by gross alterations in splenic T cell numbers, CD4 / CD8 ratios, or T and B cell activation markers, but did produce a decrease in splenocyte apoptosis. Using gld animals, Fas ligand (FasL) was implicated in the suppressive effects of S179D PRL. Circulating IgG1 and IgG2 antibody levels were increased in response to treatment with both forms of PRL, but the effects of S179D PRL were most pronounced. Cytokine changes in the popliteal lymph nodes specific to S179D PRL treatment showed an inhibition of pro-inflammatory cytokines. In conclusion, mice treated with a molecular mimic of phosphorylated prolactin showed a profound inhibition of DTH responses to Candida correlating with an absence of GM-CSF, IL-4, and IL-13 production and a marked reduction in IL-12p70 synthesis.

Original languageEnglish (US)
Pages (from-to)31-40
Number of pages10
JournalJournal of Neuroimmunology
Volume170
Issue number1-2
DOIs
StatePublished - Dec 30 2005

Fingerprint

Delayed Hypersensitivity
Candida albicans
Prolactin
Immunoglobulin G
Cytokines
T-Lymphocytes
CD4-CD8 Ratio
Fas Ligand Protein
Interleukin-13
Granulocyte-Macrophage Colony-Stimulating Factor
Candida
Interleukin-4
B-Lymphocytes
Cell Count
Lymph Nodes
Apoptosis
Antigens
Antibodies

Keywords

  • Anti-flammatory
  • Cytokines
  • Delayed type hypersensitivity
  • Fas ligand
  • Phosphorylated prolactin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin. / Guzmán, E. A.; Chen, Yen-Hao; Langowski, J. L.; De Guzman, A.; Lo, H. L.; Walter, B.; Muller, H. K.; Walker, A. M.; Owen, L. B.

In: Journal of Neuroimmunology, Vol. 170, No. 1-2, 30.12.2005, p. 31-40.

Research output: Contribution to journalArticle

Guzmán, EA, Chen, Y-H, Langowski, JL, De Guzman, A, Lo, HL, Walter, B, Muller, HK, Walker, AM & Owen, LB 2005, 'Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin', Journal of Neuroimmunology, vol. 170, no. 1-2, pp. 31-40. https://doi.org/10.1016/j.jneuroim.2005.08.009
Guzmán, E. A. ; Chen, Yen-Hao ; Langowski, J. L. ; De Guzman, A. ; Lo, H. L. ; Walter, B. ; Muller, H. K. ; Walker, A. M. ; Owen, L. B. / Abrogation of delayed type hypersensitivity response to Candida albicans produced by a molecular mimic of phosphorylated prolactin. In: Journal of Neuroimmunology. 2005 ; Vol. 170, No. 1-2. pp. 31-40.
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AU - Lo, H. L.

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