Abundance of MMPs and cysteine cathepsins in caries-affected dentin

C. M.P. Vidal, L. Tjäderhane, P. M. Scaffa, I. L. Tersariol, David Henry Pashley, H. B. Nader, F. D. Nascimento, M. R. Carrilho

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Degradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affected dentin must be higher than in intact dentin. To test this premise, we obtained 5 slices (200 μm) from 5 intact teeth and from 5 caries-affected teeth (1 slice/tooth) and individually incubated them with primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were incubated with pre-immune serum. Specimens were washed and re-incubated with the respective fluorescent secondary antibody. Collagen identification, attained by the autofluorescence capture technique, and protease localization were evaluated by multi-photon confocal microscopy. The images were analyzed with ZEN software, which also quantitatively measured the percentages of collagen and protease distribution in dentin compartments. The abundance of the test enzymes was markedly higher in caries-affected than in intact dentin. CT-B exhibited the highest percentage of co-localization with collagen, followed by MMP-9, MMP-2, and CT-K. The high expression of CTs and MMPs in caries-affected teeth indicates that those host-derived enzymes are intensely involved with caries progression.

Original languageEnglish (US)
Pages (from-to)269-274
Number of pages6
JournalJournal of Dental Research
Volume93
Issue number3
DOIs
StatePublished - Mar 1 2014

Fingerprint

Cathepsins
Metalloproteases
Dentin
Cysteine
Tooth
Cathepsin K
Cathepsin B
Peptide Hydrolases
Collagen
Antibodies
Enzymes
Photons
Confocal Microscopy
Immune Sera
Software

Keywords

  • collagen
  • dental caries
  • dentin cysteine proteases
  • fluorescent immunohistochemistry
  • metalloproteases

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Vidal, C. M. P., Tjäderhane, L., Scaffa, P. M., Tersariol, I. L., Pashley, D. H., Nader, H. B., ... Carrilho, M. R. (2014). Abundance of MMPs and cysteine cathepsins in caries-affected dentin. Journal of Dental Research, 93(3), 269-274. https://doi.org/10.1177/0022034513516979

Abundance of MMPs and cysteine cathepsins in caries-affected dentin. / Vidal, C. M.P.; Tjäderhane, L.; Scaffa, P. M.; Tersariol, I. L.; Pashley, David Henry; Nader, H. B.; Nascimento, F. D.; Carrilho, M. R.

In: Journal of Dental Research, Vol. 93, No. 3, 01.03.2014, p. 269-274.

Research output: Contribution to journalArticle

Vidal, CMP, Tjäderhane, L, Scaffa, PM, Tersariol, IL, Pashley, DH, Nader, HB, Nascimento, FD & Carrilho, MR 2014, 'Abundance of MMPs and cysteine cathepsins in caries-affected dentin', Journal of Dental Research, vol. 93, no. 3, pp. 269-274. https://doi.org/10.1177/0022034513516979
Vidal CMP, Tjäderhane L, Scaffa PM, Tersariol IL, Pashley DH, Nader HB et al. Abundance of MMPs and cysteine cathepsins in caries-affected dentin. Journal of Dental Research. 2014 Mar 1;93(3):269-274. https://doi.org/10.1177/0022034513516979
Vidal, C. M.P. ; Tjäderhane, L. ; Scaffa, P. M. ; Tersariol, I. L. ; Pashley, David Henry ; Nader, H. B. ; Nascimento, F. D. ; Carrilho, M. R. / Abundance of MMPs and cysteine cathepsins in caries-affected dentin. In: Journal of Dental Research. 2014 ; Vol. 93, No. 3. pp. 269-274.
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