Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells

Toshio Ariga, Robert K Yu, J. Neel Scarsdale, Minoru Suzuki, Yoichiro Kuroda, Hisayo Kitagawa, Tadashi Miyatake

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In a previous paper, we reported the presence of globoside as a major neutral glycolipid in PC 12 pheochromocytoma cells [Ariga, T., Macala, L. J., Saito, M., Margolis, R. K., Greene, L. A., Margolis, R. U., & Yu, R. K. (1988) Biochemistry 27, 52–58], Recently, we found that subcloned PC12h cells accumulated another unusual neutral glycolipid. In order to characterize this glycolipid, PC12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated two major neutral glycolipids, which were purified by Iatrobeads column and preparative thin-layer chromatographies. One of the glycolipids was found to be globoside, and the other had a globotriaosyl structure with an additional terminal Galα 1–3 residue. Its structure was determined by fast atom bombardment mass spectrometry, two-dimensional proton nuclear magnetic resonance spectrometry (2D NMR), permethylation study, sequential degradation with exoglycosidase, and mild acid hydrolysis to be Gal(αl-3)Gal(αl-4)Gal(β1-4)Glc(β1-1′)Cer.

Original languageEnglish (US)
Pages (from-to)5335-5340
Number of pages6
JournalBiochemistry
Volume27
Issue number14
DOIs
StatePublished - Jul 1 1988
Externally publishedYes

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Glycolipids
Pheochromocytoma
Globosides
Nuclear magnetic resonance
Fast Atom Bombardment Mass Spectrometry
Thin layer chromatography
Biochemistry
Glycoside Hydrolases
Thin Layer Chromatography
Spectrometry
Mass spectrometry
Protons
Rats
Tumors
Hydrolysis
Spectrum Analysis
Magnetic Resonance Spectroscopy
Tissue
Degradation
Atoms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ariga, T., Yu, R. K., Scarsdale, J. N., Suzuki, M., Kuroda, Y., Kitagawa, H., & Miyatake, T. (1988). Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells. Biochemistry, 27(14), 5335-5340. https://doi.org/10.1021/bi00414a058

Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells. / Ariga, Toshio; Yu, Robert K; Scarsdale, J. Neel; Suzuki, Minoru; Kuroda, Yoichiro; Kitagawa, Hisayo; Miyatake, Tadashi.

In: Biochemistry, Vol. 27, No. 14, 01.07.1988, p. 5335-5340.

Research output: Contribution to journalArticle

Ariga, T, Yu, RK, Scarsdale, JN, Suzuki, M, Kuroda, Y, Kitagawa, H & Miyatake, T 1988, 'Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells', Biochemistry, vol. 27, no. 14, pp. 5335-5340. https://doi.org/10.1021/bi00414a058
Ariga, Toshio ; Yu, Robert K ; Scarsdale, J. Neel ; Suzuki, Minoru ; Kuroda, Yoichiro ; Kitagawa, Hisayo ; Miyatake, Tadashi. / Accumulation of A Globo-series Glycolipid Having Galα 1–3 Gal In Pc12h Pheochromocytoma Cells. In: Biochemistry. 1988 ; Vol. 27, No. 14. pp. 5335-5340.
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