Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells

T. Ariga, M. Suzuki, Robert K Yu, Y. Kuroda, I. Shimada, F. Inagaki, T. Miyatake

Research output: Contribution to journalArticle

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Abstract

In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R.K., Scarsdale, J.N., Suzuki, M., Kuroda Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fucα1-2Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; B, GalNAcβ1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; C, Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; and D, Galα1-3Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M.E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M.E., Falk, K.-E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.

Original languageEnglish (US)
Pages (from-to)1516-1521
Number of pages6
JournalJournal of Biological Chemistry
Volume264
Issue number3
StatePublished - Jan 1 1989

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Glycolipids
Pheochromocytoma
Rats
Fast Atom Bombardment Mass Spectrometry
Thin layer chromatography
Biochemistry
Glycoside Hydrolases
Thin Layer Chromatography
Nuclear magnetic resonance spectroscopy
Small Intestine
Mass spectrometry
Tumors
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Tissue
Degradation
Atoms

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ariga, T., Suzuki, M., Yu, R. K., Kuroda, Y., Shimada, I., Inagaki, F., & Miyatake, T. (1989). Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells. Journal of Biological Chemistry, 264(3), 1516-1521.

Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells. / Ariga, T.; Suzuki, M.; Yu, Robert K; Kuroda, Y.; Shimada, I.; Inagaki, F.; Miyatake, T.

In: Journal of Biological Chemistry, Vol. 264, No. 3, 01.01.1989, p. 1516-1521.

Research output: Contribution to journalArticle

Ariga, T, Suzuki, M, Yu, RK, Kuroda, Y, Shimada, I, Inagaki, F & Miyatake, T 1989, 'Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells', Journal of Biological Chemistry, vol. 264, no. 3, pp. 1516-1521.
Ariga T, Suzuki M, Yu RK, Kuroda Y, Shimada I, Inagaki F et al. Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells. Journal of Biological Chemistry. 1989 Jan 1;264(3):1516-1521.
Ariga, T. ; Suzuki, M. ; Yu, Robert K ; Kuroda, Y. ; Shimada, I. ; Inagaki, F. ; Miyatake, T. / Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 3. pp. 1516-1521.
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N2 - In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R.K., Scarsdale, J.N., Suzuki, M., Kuroda Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fucα1-2Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; B, GalNAcβ1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; C, Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; and D, Galα1-3Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M.E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M.E., Falk, K.-E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.

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