TY - JOUR
T1 - Accumulation of unique globo-series glycolipids in PC 12h pheochromocytoma cells
AU - Ariga, T.
AU - Suzuki, M.
AU - Yu, Robert K
AU - Kuroda, Y.
AU - Shimada, I.
AU - Inagaki, F.
AU - Miyatake, T.
PY - 1989
Y1 - 1989
N2 - In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R.K., Scarsdale, J.N., Suzuki, M., Kuroda Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fucα1-2Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; B, GalNAcβ1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; C, Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; and D, Galα1-3Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M.E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M.E., Falk, K.-E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.
AB - In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R.K., Scarsdale, J.N., Suzuki, M., Kuroda Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fucα1-2Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; B, GalNAcβ1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; C, Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; and D, Galα1-3Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M.E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M.E., Falk, K.-E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.
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M3 - Article
C2 - 2912970
AN - SCOPUS:0024495684
SN - 0021-9258
VL - 264
SP - 1516
EP - 1521
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 3
ER -