In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R.K., Scarsdale, J.N., Suzuki, M., Kuroda Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fucα1-2Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; B, GalNAcβ1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; C, Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer; and D, Galα1-3Galα1-3Galα1-3Galα1-4Galβ1-4Glcβ1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M.E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M.E., Falk, K.-E., Hansson, G.C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Biological Chemistry|
|State||Published - 1989|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology