Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats

Michael W. Müller, Paul L McNeil, Peter Büchler, Güralp O. Ceyhan, Elke Wolf-Hieber, Guido Adler, Hans G. Beger, Markus W. Büchler, Helmut Friess

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To test the hypothesis that disruption of acinar cell membranes is the earliest event that takes place after the onset of acute pancreatitis. Methods: Cerulein and taurocholate pancreatitis were induced in rats. Furthermore, stimulation with different doses of bombesin, pilocarpine, and cerulein was performed. Five to 180 minutes after initiation of treatment, animals were killed. Disruption of cell membranes was detected by the penetration of the experimental animal's own albumin or immunoglobulin G (IgG) into acinar cells by immunocytological localization. Tissue was further analyzed by electron microscopy and electron microscopic immunostaining. Results: Animals with pancreatitis displayed significantly greater antialbumin and anti-IgG immunostaining in the cytoplasm of acinar cells and in vacuoles in comparison with controls, confirming membrane disruption. This was not detectable after stimulation with bombesin, pilocarpine, and nonsupramaximal doses of cerulein. The first changes were seen after 5 minutes of induction of pancreatitis. Results were verified by electron microscopy and electron microscopic immunohistochemistry. Conclusions: The penetration of albumin and IgG into acinar cells indicates that wounding of their plasma membrane occurs at the onset of acute pancreatitis. Disruption of the membranes could be expected to allow the influx of calcium ions, causing massive intracellular alterations, and exit of molecules, such as enzymes from acinar cells.

Original languageEnglish (US)
JournalPancreas
Volume35
Issue number4
DOIs
StatePublished - Jan 1 2007

Fingerprint

Acinar Cells
Pancreatitis
Ceruletide
Cell Membrane
Bombesin
Pilocarpine
Immunoglobulin G
Albumins
Electron Microscopy
Electrons
Taurocholic Acid
Membranes
Vacuoles
Cytoplasm
Immunohistochemistry
Ions
Calcium
Enzymes

Keywords

  • Acute pancreatitis
  • Cerulein
  • Membrane disruption
  • Rats
  • Taurocholate

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Müller, M. W., McNeil, P. L., Büchler, P., Ceyhan, G. O., Wolf-Hieber, E., Adler, G., ... Friess, H. (2007). Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats. Pancreas, 35(4). https://doi.org/10.1097/mpa.0b013e318120024c

Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats. / Müller, Michael W.; McNeil, Paul L; Büchler, Peter; Ceyhan, Güralp O.; Wolf-Hieber, Elke; Adler, Guido; Beger, Hans G.; Büchler, Markus W.; Friess, Helmut.

In: Pancreas, Vol. 35, No. 4, 01.01.2007.

Research output: Contribution to journalArticle

Müller, MW, McNeil, PL, Büchler, P, Ceyhan, GO, Wolf-Hieber, E, Adler, G, Beger, HG, Büchler, MW & Friess, H 2007, 'Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats', Pancreas, vol. 35, no. 4. https://doi.org/10.1097/mpa.0b013e318120024c
Müller MW, McNeil PL, Büchler P, Ceyhan GO, Wolf-Hieber E, Adler G et al. Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats. Pancreas. 2007 Jan 1;35(4). https://doi.org/10.1097/mpa.0b013e318120024c
Müller, Michael W. ; McNeil, Paul L ; Büchler, Peter ; Ceyhan, Güralp O. ; Wolf-Hieber, Elke ; Adler, Guido ; Beger, Hans G. ; Büchler, Markus W. ; Friess, Helmut. / Acinar cell membrane disruption is an early event in experimental acute pancreatitis in rats. In: Pancreas. 2007 ; Vol. 35, No. 4.
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