Activation of urocanase from Pseudomonas putida by electronically excited triplet species

R. C. Venema, D. H. Hug

Research output: Contribution to journalArticle

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Abstract

Urocanase from Pseudomonas putida becomes inactive in growing and resting cells and, as shown previously, is activated by the direct absorption of ultraviolet light. In this study, we describe the activation of urocanase by energy transfer from triplet indole-3-aldehyde, generated in the peroxidase-catalyzed aerobic oxidation of indole-3-acetic acid. The activation was time-, temperature-, and pH-dependent. The involvement of reactive oxygen intermediates was excluded by the lack of effect of appropriate quenchers and traps. Triplet quenchers, in contrast, reduced the level of activation. Photoexcited rose bengal, a triplet species of a different nature and origin, was also effective in promoting activation. These results demonstrate a potential mechanism of urocanase regulation not dependent on an environmental source of light, but rather brought about by an enzymically generated excited species.

Original languageEnglish (US)
Pages (from-to)12190-12193
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number22
StatePublished - Dec 1 1985

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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