Activity-dependent NFATc3 nuclear accumulation in pericytes from cortical parenchymal microvessels

Jessica Andrea Filosa, Mark T. Nelson, Laura V. Gonzalez Bosc

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The calcium-dependent transcription factor NFATc3, which is a member of the nuclear factor of activated T cells (NFAT) family of transcription factors, is critical for embryonic vascular development and differentiation. Despite its potential importance, nothing is known about NFATc3 regulation in the brain microcirculation. In the present study, we sought to investigate the role that glutamate, possibly through astrocytic communication, plays in the control of NFATc3 regulation in pericytes from parenchymal microvessels. Coronal cortical slices from neonatal rats were subjected to electrical field stimulation or were treated with the metabotropic glutamate receptor agonist (±)-1- aminocyclopentane-trans-1,3-dicarboxylic acid (t-ACPD). NFATc3, glial fibrillary acidic protein (an astrocyte-specific marker), and platelet-derived growth factor-β-receptor (a pericyte-specific marker) were detected by immunofluorescence. Electrical field stimulation induced NFATc3 nuclear accumulation in pericytes. This response was dependent on neuronal activity and group I metabotropic glutamate receptor (mGluR) activation. In addition, t-ACPD significantly increased NFATc3 nuclear accumulation in both astrocytes and pericytes. NFATc3 nuclear accumulation in pericytes was prevented when astrocytic function was abolished with the gliotoxin L-α-aminoadipate or by the inhibition of calcineurin, cyclooxygenase, and nitric oxide synthase. This is the first study to report NFATc3 expression in pericytes from parenchymal microvessels and in astrocytes from native tissue. Our results suggest a model by which glutamate, via mGluR activation, may regulate gene transcription in pluripotent vascular pericytes.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume293
Issue number6
DOIs
StatePublished - Dec 1 2007
Externally publishedYes

Fingerprint

Pericytes
Microvessels
Astrocytes
Metabotropic Glutamate Receptors
Electric Stimulation
Blood Vessels
Glutamic Acid
Transcription Factors
Gliotoxin
NFATC Transcription Factors
Excitatory Amino Acid Agonists
Platelet-Derived Growth Factor Receptors
Dicarboxylic Acids
transcription factor NF-AT c3
Calcineurin
Glial Fibrillary Acidic Protein
Microcirculation
Prostaglandin-Endoperoxide Synthases
Nitric Oxide Synthase
Embryonic Development

Keywords

  • Astrocytes
  • Brain cortex
  • Calcium
  • Neuronal activity
  • Rat

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Activity-dependent NFATc3 nuclear accumulation in pericytes from cortical parenchymal microvessels. / Filosa, Jessica Andrea; Nelson, Mark T.; Gonzalez Bosc, Laura V.

In: American Journal of Physiology - Cell Physiology, Vol. 293, No. 6, 01.12.2007.

Research output: Contribution to journalArticle

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