Activity patterns of biotransformation enzymes in juvenile chickens after in ovo dosage of PCB126

Peter van den Hurk, Faith E. Wiley, Emma T. Lavoie, Keith A. Grasman, William W. Bowerman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Little is known about the correlations between biotransformation enzymes in juvenile birds after exposure to environmental toxicants like PCBs. In this study eggs of domestic chicken (Gallus domesticus) were dosed with PCB126 in concentrations of 0.175-0.325 ng/g egg weight. Liver subcellular fractions were analyzed for activities of Phase 1 and Phase 2 biotransformation enzymes 2 and 5 weeks post-hatch. Ethoxyresorufin O-deethylase (EROD) activity was increased in both the 2-week and 5-week samples. Glutathione-S-transferase (GST) activity was increased in the 2-week samples only, but the 5-week samples showed an overall much higher GST activity, probably as a result of a still developing enzyme expression in maturing chickens. The same pattern was seen in the phenol-type UDP-glucuronosyltransferase (UGT) activity of the control animals. The week two samples showed a positive dose-response relationship for the UGT activity, but after 5 weeks this was reversed, possibly caused by inhibition of hydroxylated PCB metabolites. Phenol-type sulfotransferase (SULT) activities were not significantly correlated with time or dose. There was a strong positive regression between the Ah-receptor mediated EROD and UGT activities. The EROD activities were also positively correlated to the GST activities. Most interesting was a negative correlation between the UGT and SULT activities: an inhibited UGT activity appeared to be compensated by an increased SULT activity.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume146
Issue number3
DOIs
StatePublished - Sep 2007
Externally publishedYes

Keywords

  • Cytochrome P450-1A
  • Enzyme activity
  • Enzyme interactions
  • Ethoxyresorufin O-deethylase
  • Glutathione S-transferase
  • Polychlorinated biphenyls
  • Sulfotransferase
  • UDP-glucuronosyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

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