Activity patterns of biotransformation enzymes in juvenile chickens after in ovo dosage of PCB126

Peter van den Hurk, Faith E. Wiley, Emma T. Lavoie, Keith A. Grasman, William W. Bowerman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Little is known about the correlations between biotransformation enzymes in juvenile birds after exposure to environmental toxicants like PCBs. In this study eggs of domestic chicken (Gallus domesticus) were dosed with PCB126 in concentrations of 0.175-0.325 ng/g egg weight. Liver subcellular fractions were analyzed for activities of Phase 1 and Phase 2 biotransformation enzymes 2 and 5 weeks post-hatch. Ethoxyresorufin O-deethylase (EROD) activity was increased in both the 2-week and 5-week samples. Glutathione-S-transferase (GST) activity was increased in the 2-week samples only, but the 5-week samples showed an overall much higher GST activity, probably as a result of a still developing enzyme expression in maturing chickens. The same pattern was seen in the phenol-type UDP-glucuronosyltransferase (UGT) activity of the control animals. The week two samples showed a positive dose-response relationship for the UGT activity, but after 5 weeks this was reversed, possibly caused by inhibition of hydroxylated PCB metabolites. Phenol-type sulfotransferase (SULT) activities were not significantly correlated with time or dose. There was a strong positive regression between the Ah-receptor mediated EROD and UGT activities. The EROD activities were also positively correlated to the GST activities. Most interesting was a negative correlation between the UGT and SULT activities: an inhibited UGT activity appeared to be compensated by an increased SULT activity.

Original languageEnglish (US)
Pages (from-to)301-307
Number of pages7
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume146
Issue number3
DOIs
StatePublished - Sep 1 2007
Externally publishedYes

Fingerprint

Glucuronosyltransferase
Biotransformation
Cytochrome P-450 CYP1A1
Chickens
Glutathione Transferase
Sulfotransferases
Polychlorinated Biphenyls
Enzymes
Arylsulfotransferase
Hatches
Subcellular Fractions
Environmental Exposure
Birds
Metabolites
Liver
Eggs
Ovum
Animals
Weights and Measures

Keywords

  • Cytochrome P450-1A
  • Enzyme activity
  • Enzyme interactions
  • Ethoxyresorufin O-deethylase
  • Glutathione S-transferase
  • Polychlorinated biphenyls
  • Sulfotransferase
  • UDP-glucuronosyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Activity patterns of biotransformation enzymes in juvenile chickens after in ovo dosage of PCB126. / Hurk, Peter van den; Wiley, Faith E.; Lavoie, Emma T.; Grasman, Keith A.; Bowerman, William W.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 146, No. 3, 01.09.2007, p. 301-307.

Research output: Contribution to journalArticle

Hurk, Peter van den ; Wiley, Faith E. ; Lavoie, Emma T. ; Grasman, Keith A. ; Bowerman, William W. / Activity patterns of biotransformation enzymes in juvenile chickens after in ovo dosage of PCB126. In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2007 ; Vol. 146, No. 3. pp. 301-307.
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