Adhesion development and the expression of endothelial nitric oxide synthase

David M. Svinarich, Fadi M. Zaher, Lena Holmdahl, Nasser Chegini, Bernard Gonik, Michael Peter Diamond

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalInfectious Diseases in Obstetrics and Gynecology
Volume9
Issue number2
DOIs
StatePublished - Jan 1 2001

Fingerprint

Nitric Oxide Synthase Type III
Tissue Adhesions
Messenger RNA
Peritoneum
Subcutaneous Tissue
Actins
Maintenance
Skin
Vasodilator Agents
Reverse Transcription
Nitric Oxide
Thrombosis
RNA
Polymerase Chain Reaction

Keywords

  • Adhesions
  • QRT-PCR
  • Transcriptional Regulation
  • eNos

ASJC Scopus subject areas

  • Dermatology
  • Obstetrics and Gynecology
  • Infectious Diseases

Cite this

Adhesion development and the expression of endothelial nitric oxide synthase. / Svinarich, David M.; Zaher, Fadi M.; Holmdahl, Lena; Chegini, Nasser; Gonik, Bernard; Diamond, Michael Peter.

In: Infectious Diseases in Obstetrics and Gynecology, Vol. 9, No. 2, 01.01.2001, p. 113-116.

Research output: Contribution to journalArticle

Svinarich, David M. ; Zaher, Fadi M. ; Holmdahl, Lena ; Chegini, Nasser ; Gonik, Bernard ; Diamond, Michael Peter. / Adhesion development and the expression of endothelial nitric oxide synthase. In: Infectious Diseases in Obstetrics and Gynecology. 2001 ; Vol. 9, No. 2. pp. 113-116.
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