Adhesion development and the expression of endothelial nitric oxide synthase

David M. Svinarich, Fadi M. Zaher, Lena Holmdahl, Nasser Chegini, Bernard Gonik, Michael P. Diamond

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions. Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels. Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl. Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

Original languageEnglish (US)
Pages (from-to)113-116
Number of pages4
JournalInfectious Diseases in Obstetrics and Gynecology
Volume9
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

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Keywords

  • Adhesions
  • QRT-PCR
  • Transcriptional Regulation
  • eNos

ASJC Scopus subject areas

  • Dermatology
  • Obstetrics and Gynecology
  • Infectious Diseases

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