Adjunctive application of chlorhexidine and ethanol-wet bonding on durability of bonds to sound and caries-affected dentine

Manikandan Ekambaram, Cynthia Kar Yung Yiu, Jukka Pekka Matinlinna, Nigel Martyn King, Franklin Russell Tay

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives To examine the effect of adjunctive application of chlorhexidine (CHX) and ethanol-wet bonding (EWB) on bond durability and nanoleakage of hydrophobic adhesive to sound (SD) and caries-affected dentine (CAD). Methods Dentine surfaces of molars were etched after caries removal and randomly allocated to four groups (n = 12). In Groups 1 and 2, dentine surfaces were saturated with either 2 ml of 100% ethanol or 2 ml of ethanol with 2% CHX for 60 s. In Groups 3 and 4, dentine surfaces were saturated with either 15 μL of distilled water or 15 μL of distilled water with 2% CHX for 60 s. Two coats of primer, followed by neat resin were applied and light-cured for 40 s. Resin composite build-ups were placed and bonded specimens were sectioned for bond strength testing after 24 h and 12 months' storage in artificial saliva. Bond strength data were analyzed using 3-way ANOVA and SNK tests. Interfacial nanoleakage was evaluated after 24 h and 12 months using a field-emission scanning electron microscopy and data were analyzed using Kruskal-Wallis test. Results Significant differences were observed for the three factors: "substrate" (p < 0.001), "rewetting agents" (p < 0.001) and "time" (p < 0.001) on bond strength. Incorporation of 2% CHX to EWB preserved bond strength to SD and CAD and reduced interfacial nanoleakage after 12 months. Incorporation of 2% CHX to WWB also preserved bond strength to SD after ageing. Conclusions Incorporation of chlorhexidine to ethanol-wet bonding has an interaction effect on preservation of bond durability to sound and caries-affected dentine. Clinical significance Incorporation of chlorhexidine to ethanol-wet bonding with hydrophobic adhesive enhances the success rate of aesthetic bonded restorations.

Original languageEnglish (US)
Pages (from-to)709-719
Number of pages11
JournalJournal of Dentistry
Volume42
Issue number6
DOIs
StatePublished - Jun 2014

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Chlorhexidine
Dentin
Ethanol
Adhesives
Artificial Saliva
Water
Composite Resins
Esthetics
Electron Scanning Microscopy
Analysis of Variance
Light

Keywords

  • Acid etching
  • Caries-affected dentine
  • Chlorhexidine
  • Dentine bonding
  • Ethanol-wet bonding
  • Hydrophobic adhesives

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Adjunctive application of chlorhexidine and ethanol-wet bonding on durability of bonds to sound and caries-affected dentine. / Ekambaram, Manikandan; Yiu, Cynthia Kar Yung; Matinlinna, Jukka Pekka; King, Nigel Martyn; Tay, Franklin Russell.

In: Journal of Dentistry, Vol. 42, No. 6, 06.2014, p. 709-719.

Research output: Contribution to journalArticle

Ekambaram, Manikandan ; Yiu, Cynthia Kar Yung ; Matinlinna, Jukka Pekka ; King, Nigel Martyn ; Tay, Franklin Russell. / Adjunctive application of chlorhexidine and ethanol-wet bonding on durability of bonds to sound and caries-affected dentine. In: Journal of Dentistry. 2014 ; Vol. 42, No. 6. pp. 709-719.
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T1 - Adjunctive application of chlorhexidine and ethanol-wet bonding on durability of bonds to sound and caries-affected dentine

AU - Ekambaram, Manikandan

AU - Yiu, Cynthia Kar Yung

AU - Matinlinna, Jukka Pekka

AU - King, Nigel Martyn

AU - Tay, Franklin Russell

PY - 2014/6

Y1 - 2014/6

N2 - Objectives To examine the effect of adjunctive application of chlorhexidine (CHX) and ethanol-wet bonding (EWB) on bond durability and nanoleakage of hydrophobic adhesive to sound (SD) and caries-affected dentine (CAD). Methods Dentine surfaces of molars were etched after caries removal and randomly allocated to four groups (n = 12). In Groups 1 and 2, dentine surfaces were saturated with either 2 ml of 100% ethanol or 2 ml of ethanol with 2% CHX for 60 s. In Groups 3 and 4, dentine surfaces were saturated with either 15 μL of distilled water or 15 μL of distilled water with 2% CHX for 60 s. Two coats of primer, followed by neat resin were applied and light-cured for 40 s. Resin composite build-ups were placed and bonded specimens were sectioned for bond strength testing after 24 h and 12 months' storage in artificial saliva. Bond strength data were analyzed using 3-way ANOVA and SNK tests. Interfacial nanoleakage was evaluated after 24 h and 12 months using a field-emission scanning electron microscopy and data were analyzed using Kruskal-Wallis test. Results Significant differences were observed for the three factors: "substrate" (p < 0.001), "rewetting agents" (p < 0.001) and "time" (p < 0.001) on bond strength. Incorporation of 2% CHX to EWB preserved bond strength to SD and CAD and reduced interfacial nanoleakage after 12 months. Incorporation of 2% CHX to WWB also preserved bond strength to SD after ageing. Conclusions Incorporation of chlorhexidine to ethanol-wet bonding has an interaction effect on preservation of bond durability to sound and caries-affected dentine. Clinical significance Incorporation of chlorhexidine to ethanol-wet bonding with hydrophobic adhesive enhances the success rate of aesthetic bonded restorations.

AB - Objectives To examine the effect of adjunctive application of chlorhexidine (CHX) and ethanol-wet bonding (EWB) on bond durability and nanoleakage of hydrophobic adhesive to sound (SD) and caries-affected dentine (CAD). Methods Dentine surfaces of molars were etched after caries removal and randomly allocated to four groups (n = 12). In Groups 1 and 2, dentine surfaces were saturated with either 2 ml of 100% ethanol or 2 ml of ethanol with 2% CHX for 60 s. In Groups 3 and 4, dentine surfaces were saturated with either 15 μL of distilled water or 15 μL of distilled water with 2% CHX for 60 s. Two coats of primer, followed by neat resin were applied and light-cured for 40 s. Resin composite build-ups were placed and bonded specimens were sectioned for bond strength testing after 24 h and 12 months' storage in artificial saliva. Bond strength data were analyzed using 3-way ANOVA and SNK tests. Interfacial nanoleakage was evaluated after 24 h and 12 months using a field-emission scanning electron microscopy and data were analyzed using Kruskal-Wallis test. Results Significant differences were observed for the three factors: "substrate" (p < 0.001), "rewetting agents" (p < 0.001) and "time" (p < 0.001) on bond strength. Incorporation of 2% CHX to EWB preserved bond strength to SD and CAD and reduced interfacial nanoleakage after 12 months. Incorporation of 2% CHX to WWB also preserved bond strength to SD after ageing. Conclusions Incorporation of chlorhexidine to ethanol-wet bonding has an interaction effect on preservation of bond durability to sound and caries-affected dentine. Clinical significance Incorporation of chlorhexidine to ethanol-wet bonding with hydrophobic adhesive enhances the success rate of aesthetic bonded restorations.

KW - Acid etching

KW - Caries-affected dentine

KW - Chlorhexidine

KW - Dentine bonding

KW - Ethanol-wet bonding

KW - Hydrophobic adhesives

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