ALFALFA SEEDLINGS GROWN OUTDOORS ARE MORE RESISTANT TO UV‐INDUCED DNA DAMAGE THAN PLANTS GROWN IN A UV‐FREE ENVIRONMENTAL CHAMBER

Shinnosuke Takayanagi, John G. Trunk, John C. Sutherland, Betsy M. Sutherland

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Abstract The relative UV sensitivities of alfalfa seedlings grown outdoors versus plants grown in a growth chamber under UV‐filtered cool white fluorescent bulbs have been determined using three criteria: (1) level of endogenous DNA damage as sites for the UV endonuclease from Micrococcus luteus. (2) susceptibility to pyrimidine dimer induction by a UV challenge exposure and (3) ability to repair UV‐induced damage. We find that outdoor‐grown plants contain approximately equal frequencies of endogenous DNA damages, are less susceptible to dimer induction by a challenge exposure of broad‐spectrum UV and photorepair dimers more rapidly than plants grown in an environmental chamber under cool white fluorescent lamps plus a filter that removes most UV radiation. These data suggest that plants grown in a natural environment would be less sensitive to UVB‐induced damage than would be predicted on the basis of studies on plants grown under minimum UV.

Original languageEnglish (US)
Pages (from-to)363-367
Number of pages5
JournalPhotochemistry and Photobiology
Volume60
Issue number4
DOIs
StatePublished - Jan 1 1994

Fingerprint

alfalfa
Environmental chambers
test chambers
Medicago sativa
Seedlings
Dimers
DNA Damage
deoxyribonucleic acid
damage
Pyrimidine Dimers
Fluorescent lamps
DNA
dimers
Ultraviolet radiation
induction
Repair
phytotrons
bulbs
Micrococcus luteus
pyrimidines

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

ALFALFA SEEDLINGS GROWN OUTDOORS ARE MORE RESISTANT TO UV‐INDUCED DNA DAMAGE THAN PLANTS GROWN IN A UV‐FREE ENVIRONMENTAL CHAMBER. / Takayanagi, Shinnosuke; Trunk, John G.; Sutherland, John C.; Sutherland, Betsy M.

In: Photochemistry and Photobiology, Vol. 60, No. 4, 01.01.1994, p. 363-367.

Research output: Contribution to journalArticle

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