Alteration in angiogenic and anti-angiogenic forms of vascular endothelial growth factor-A in skeletal muscle of patients with intermittent claudication following exercise training

W. Schuyler Jones, Brian D. Duscha, Jennifer L. Robbins, Natasha N. Duggan, Judith G. Regensteiner, William E. Kraus, William R. Hiatt, Ayotunde O. Dokun, Brian H. Annex

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

The aims of this study were twofold: (1) to identify whether peripheral artery disease (PAD) patients had increased muscle concentration of angiogenic VEGF-A, anti-angiogenic VEGF165b or VEGF receptor 1 (VEGF-R1) when compared with control subjects, and (2) to evaluate whether exercise training in PAD patients was associated with changes in muscle concentration of VEGF-A, VEGF165b or VEGF-R1. At baseline, 22 PAD and 30 control subjects underwent gastrocnemius muscle biopsy. Twelve PAD patients were treated with supervised exercise training (SET) and underwent muscle biopsy after 3 weeks and 12 weeks of training and had sufficient tissue to measure VEGF-A, VEGF165b and VEGF-R1 concentrations in skeletal muscle lysates by ELISA. Muscle concentrations of VEGF-A and VEGF165b were similar in PAD patients versus controls at baseline. At both time points after the start of SET, VEGF-A levels decreased and there was a trend towards increased VEGF165b concentrations. At baseline, VEGF-R1 concentrations were lower in PAD patients when compared with controls but did not change after SET. Skeletal muscle concentrations of VEGF-A are not different in PAD patients when compared with controls at baseline. SET is associated with a significant reduction in VEGF-A levels and a trend towards increased VEGF165b levels. These somewhat unexpected findings suggest that further investigation into the mechanism of vascular responses to exercise training in PAD patients is warranted.

Original languageEnglish (US)
Pages (from-to)94-100
Number of pages7
JournalVascular Medicine (United Kingdom)
Volume17
Issue number2
DOIs
StatePublished - Apr 1 2012
Externally publishedYes

Keywords

  • angiogenesis
  • peripheral artery disease
  • vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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