Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease

Jay M. Bolnick, Hamid Reza Kohan-Ghadr, Rani Fritz, Alan D. Bolnick, Brian A. Kilburn, Michael P. Diamond, D. Randall Armant, Sascha Drewlo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A contributing factor to poor placental perfusion, leading to intrauterine growth restriction and preeclampsia, is the failure of invading extravillous trophoblast (EVT) cells to remodel the maternal uterine arteries during the first and second trimesters of pregnancy. Noninvasive assessment of EVT cells in ongoing pregnancies is possible beginning three weeks after conception, using trophoblast retrieval and isolation from the cervix (TRIC). Seven proteins were semi-quantified by immunofluorescence microscopy in EVT cells obtained between gestational weeks 6 and 20 from pregnancies with normal outcomes (N = 29) and those with intrauterine growth restriction or preeclampsia (N = 12). Significant differences were measured in expression of PAPPA, FLT1, ENG, AFP, PGF, and LGALS14, but not LGALS13 or the lineage marker KRT7. These findings provide for the first time direct evidence of pathology-associated protein dysregulation in EVT cells during early placentation. The TRIC platform provides a novel approach to acquire molecular signatures of EVT cells that can be correlated with pregnancy outcome.

Original languageEnglish (US)
Article number32382
JournalScientific Reports
Volume6
DOIs
StatePublished - Sep 23 2016

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Trophoblasts
Biomarkers
Pre-Eclampsia
Cervix Uteri
Pregnancy
Placentation
Uterine Artery
Prostaglandins F
Second Pregnancy Trimester
First Pregnancy Trimester
Pregnancy Outcome
Growth
Fluorescence Microscopy
Proteins
Perfusion
Mothers
Pathology

ASJC Scopus subject areas

  • General

Cite this

Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease. / Bolnick, Jay M.; Kohan-Ghadr, Hamid Reza; Fritz, Rani; Bolnick, Alan D.; Kilburn, Brian A.; Diamond, Michael P.; Armant, D. Randall; Drewlo, Sascha.

In: Scientific Reports, Vol. 6, 32382, 23.09.2016.

Research output: Contribution to journalArticle

Bolnick, Jay M. ; Kohan-Ghadr, Hamid Reza ; Fritz, Rani ; Bolnick, Alan D. ; Kilburn, Brian A. ; Diamond, Michael P. ; Armant, D. Randall ; Drewlo, Sascha. / Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease. In: Scientific Reports. 2016 ; Vol. 6.
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