Altered calcium sequestration by subcellular fractions of vascular smooth muscle from spontaneously hypertensive rats

R Clinton Webb, Ramesh C. Bhalla

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

The calcium sequestration characteristics of microsomal and mitochondrial fractions prepared from aortae of spontaneously hypertensive (SHR), Kyoto Wistar normotensive (NWR), and normotensive Sprague-Dawley (NSDR) rats were studied. Calcium uptake by the microsomal vesicles of SHR was significantly lower as compared to NWR and NSDR. However, mitochondria isolated from SHR aortae did not differ from NWR in their calcium uptake characteristics. Microsomal vesicles of NSDR aortae accumulated consistently more calcium than NWR. These findings illustrate the significance of relevant controls for SHR. Calcium-dependent ATPase activity in the microsomal fraction was significantly increased in SHR as compared to NWR. This may indicate a compensatory phenomenon on the part of the smooth muscle cell to increase Ca2+ uptake by the microsomal vesicles. These results suggest that a decreased ability to sequester calcium by the microsomal fraction of vascular smooth muscle of SHR may result in altered calcium distribution in the muscle cell.

Original languageEnglish (US)
Pages (from-to)651-661
Number of pages11
JournalJournal of Molecular and Cellular Cardiology
Volume8
Issue number8
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

Fingerprint

Subcellular Fractions
Inbred SHR Rats
Vascular Smooth Muscle
Calcium
Aorta
Calcium-Transporting ATPases
Muscle Cells
Smooth Muscle Myocytes
Sprague Dawley Rats
Mitochondria

Keywords

  • Aortic smooth muscle
  • Calcium sequestration
  • Microsomal vesicles
  • Mitochondria
  • Spontaneously hypertensive rats
  • Subcellular calcium distribution

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Altered calcium sequestration by subcellular fractions of vascular smooth muscle from spontaneously hypertensive rats. / Webb, R Clinton; Bhalla, Ramesh C.

In: Journal of Molecular and Cellular Cardiology, Vol. 8, No. 8, 01.01.1976, p. 651-661.

Research output: Contribution to journalArticle

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N2 - The calcium sequestration characteristics of microsomal and mitochondrial fractions prepared from aortae of spontaneously hypertensive (SHR), Kyoto Wistar normotensive (NWR), and normotensive Sprague-Dawley (NSDR) rats were studied. Calcium uptake by the microsomal vesicles of SHR was significantly lower as compared to NWR and NSDR. However, mitochondria isolated from SHR aortae did not differ from NWR in their calcium uptake characteristics. Microsomal vesicles of NSDR aortae accumulated consistently more calcium than NWR. These findings illustrate the significance of relevant controls for SHR. Calcium-dependent ATPase activity in the microsomal fraction was significantly increased in SHR as compared to NWR. This may indicate a compensatory phenomenon on the part of the smooth muscle cell to increase Ca2+ uptake by the microsomal vesicles. These results suggest that a decreased ability to sequester calcium by the microsomal fraction of vascular smooth muscle of SHR may result in altered calcium distribution in the muscle cell.

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