Altered expressions of VEGF mRNA splice variants during progression of uterine-peritoneal adhesions in the rat

Ujjwal K. Rout, Koshy Oommen, Michael Peter Diamond

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

PROBLEM: Postoperative pelvic adhesions contribute to infertility, pelvic pain, bowel obstruction, and difficult reoperative procedures. METHOD OF STUDY: In the present study, a rat uterine-peritoneal adhesion model was developed to study the progression of adhesion formation during a course of 7 days following pelvic surgery. The distal 1 cm of each uterine horn and its adjacent peritoneum was abraded by six scratches with a scalpel blade, producing punctate bleeding. The scratched portion of uterine horn and the peritoneum was then held with Vicryl 3-0 to promote adhesion. The uterine tissue and the portion of peritoneum, held with suture, were then excised from a group of four rats, each at 6, 12, 24, 48, 72 hr and 5 and 7 days following surgery. Total RNA was isolated from these tissues and the expression pattern of different splice variants of vascular endothelial growth factors (VEGF) was examined using relative abundance reverse transcriptase polymerase chain reaction (RA-RT-PCR) method. RESULTS: Three known splice variants of VEGF mRNA (VEGF120, VBGF164 and VEGF188), as well as an additional band (~510 bp), were amplified from these tissues. The relative abundance of known VEGF isoforms demonstrated altered expression during adhesion progression. When compared with noninjured uterine tissues, VEGF120 and VEGF188 demonstrated up-regulation during early stages of adhesion formation, whereas VEGF164 rather demonstrated down-regulation 24 and 48 hr following surgery. CONCLUSIONS: The up-regulation of VEGF isoforms during the progression of uterine-peritoneal adhesion may be a compensatory mechanism regulating angiogenesis in order to provide nutrients and oxygen to the injured tissues.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume43
Issue number5
StatePublished - May 1 2000
Externally publishedYes

Fingerprint

Vascular Endothelial Growth Factors
Peritoneum
Messenger RNA
Protein Isoforms
Up-Regulation
Polyglactin 910
Pelvic Pain
Reverse Transcriptase Polymerase Chain Reaction
Ambulatory Surgical Procedures
Infertility
Sutures
Down-Regulation
RNA
Hemorrhage
Oxygen
Food

Keywords

  • mRNA
  • Pelvic-adhesions
  • Reverse transcriptase polymerase chain reaction
  • Wound-healing

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Altered expressions of VEGF mRNA splice variants during progression of uterine-peritoneal adhesions in the rat. / Rout, Ujjwal K.; Oommen, Koshy; Diamond, Michael Peter.

In: American Journal of Reproductive Immunology, Vol. 43, No. 5, 01.05.2000, p. 299-304.

Research output: Contribution to journalArticle

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