Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein

Gurmukh Singh, Sikandar L. Katyal, William E. Brown, Stephen Phillips, Amy L. Kennedy, James Anthony, Nicholas Squeglia

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

A human lung cDNA expression library was screened by using a rabbit antiserum specific for a human Clara cell 10 kDa protein. The cDNA from two positive clones was sequenced by the dideoxy chain termination method. The nucleotide and primary amino-acid sequence deduced therefrom are presented. The N-terminal amino-acid sequence of the Clara cell 10 kDa protein, purified from bronchoalveolar lavage, was also determined. The deduced and experimentally determined sequences were identical where data for both were available. From the amino-acid composition, deduced and experimentally determined amino-acid sequences, it was determined that the 10 kDa protein in bronchoalveolar lavage consists of two identical 70-amino-acid long polypeptide chains joined by two cystine residues. The size of mRNA for the protein was found to be about 0.6 kb and the monomeric nascent protein, obtained by in vitro translation of lung mRNA was about 7.3 kDa in size. The 10 kDa protein recovered from bronchoalveolar lavage has 61% sequence identity with rabbit uteroglobin, the two proteins have common predicted secondary structures with marked surface differences when comparing predicted and actual structure determined by X-ray diffraction. The differences imply similarity of structure but, not identity of function.

Original languageEnglish (US)
Pages (from-to)329-337
Number of pages9
JournalBBA - Gene Structure and Expression
Volume950
Issue number3
DOIs
StatePublished - Sep 7 1988
Externally publishedYes

Fingerprint

Nucleotides
Complementary DNA
Amino Acids
Bronchoalveolar Lavage
Proteins
Amino Acid Sequence
Uteroglobin
Rabbits
Lung
Messenger RNA
Cystine
Protein Biosynthesis
Gene Library
X-Ray Diffraction
Immune Sera
Clone Cells
X ray diffraction
Peptides
Chemical analysis

Keywords

  • (Clara cell)
  • Amino acid sequence
  • Lung protein
  • Protein, 10 kDa
  • Secondary structure prediction
  • cDNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Singh, G., Katyal, S. L., Brown, W. E., Phillips, S., Kennedy, A. L., Anthony, J., & Squeglia, N. (1988). Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein. BBA - Gene Structure and Expression, 950(3), 329-337. https://doi.org/10.1016/0167-4781(88)90129-7

Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein. / Singh, Gurmukh; Katyal, Sikandar L.; Brown, William E.; Phillips, Stephen; Kennedy, Amy L.; Anthony, James; Squeglia, Nicholas.

In: BBA - Gene Structure and Expression, Vol. 950, No. 3, 07.09.1988, p. 329-337.

Research output: Contribution to journalArticle

Singh, G, Katyal, SL, Brown, WE, Phillips, S, Kennedy, AL, Anthony, J & Squeglia, N 1988, 'Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein', BBA - Gene Structure and Expression, vol. 950, no. 3, pp. 329-337. https://doi.org/10.1016/0167-4781(88)90129-7
Singh, Gurmukh ; Katyal, Sikandar L. ; Brown, William E. ; Phillips, Stephen ; Kennedy, Amy L. ; Anthony, James ; Squeglia, Nicholas. / Amino-acid and cDNA nucleotide sequences of human Clara cell 10 kDa protein. In: BBA - Gene Structure and Expression. 1988 ; Vol. 950, No. 3. pp. 329-337.
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