An animal model of chronic coronary stenosis resulting in hibernating myocardium

H. Bolukoglu, A. J. Liedtke, S. H. Nellis, A. M. Eggleston, R. Subramanian, B. Renstrom

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72 Scopus citations

Abstract

An experimental animal model of hibernating myocardium is presented. Sixteen animals were initially prepared of which seven were selected for final review. Hearts were instrumented in two separate surgical procedures such that maximum phasic flow velocity in the left anterior descending (LAD) coronary artery was reduced by 50% and followed over 1 wk. Regional shortening declined at 1 wk to 62% of aerobic values (P < 0.048) and did not improve over 2 h reperfusion. Metabolic determinations, obtained after 1 wk of coronary stenosis and immediately sampled before and after release of the LAD flow constrictor, showed no evidence of acidosis, hypercarbia, or an inability to extract oxygen at the tissue level. Thereafter, during the 2-h reperfusion period, hearts were able to respond to dobutamine (10 μg/kg infusion over 1 min) challenge with an appropriate shift in an endsystolic length estimate of contractility. Mitochondrial respiration at the conclusion of the studies in the reperfused bed demonstrated near normal recovery compared with aerobic values. None of the seven hearts showed gross evidence of infarction and only one heart was noted to have a few microfocal changes of healing infarction. Thus a new model of coronary stenosis is presented, which affected substantial reductions in mechanical function consistent with the concepts of hibernating myocardium. These mechanical events were not associated with marked metabolic abnormalities, reflecting advanced ischemia or mitochondrial dysfunction and could be transiently improved with inotropic stimuli. This model may prove beneficial as a tool in understanding mechanistic events underlying the hibernating heart.

Original languageEnglish (US)
Pages (from-to)H20-H29
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume263
Issue number1 32-1
DOIs
StatePublished - 1992

Keywords

  • dobutamine challenge
  • mechanical dysfunction
  • mitochondrial respiration
  • reactive hyperemia

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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    Bolukoglu, H., Liedtke, A. J., Nellis, S. H., Eggleston, A. M., Subramanian, R., & Renstrom, B. (1992). An animal model of chronic coronary stenosis resulting in hibernating myocardium. American Journal of Physiology - Heart and Circulatory Physiology, 263(1 32-1), H20-H29. https://doi.org/10.1152/ajpheart.1992.263.1.h20