An avocado constituent, persenone A, suppresses expression of inducible forms of nitric oxide synthase and cyclooxygenase in macrophages, and hydrogen peroxide generation in mouse skin

Oe Kyung Kim, Akira Murakami, Daisuke Takahashi, Yoshimasa Nakamura, Koji Torikai, Ha Won Kim, Hajime Ohigashi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We investigated the suppressive effects of an avocado constituent, persenone A, on lipopolysaccharide- and interferon-γ-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in a mouse macrophage cell line RAW 264.7. Persenone A at concentration of 20 μM almost completely suppressed both iNOS and COX-2 protein expression. In mouse skin, double treatments with persenone A (810 nmol) significantly suppressed double 12-O-tetradecanoylphorbol-13-acetate (TPA, 8.1 nmol) application-induced hydrogen peroxide (H2O2) generation. Treatment with persenone A before the second TPA treatment was sufficient to inhibit H2O2 generation, while the first treatment was not. This study thus suggests that persenone A is a possible agent to prevent inflammation-associated diseases including cancer.

Original languageEnglish (US)
Pages (from-to)2504-2507
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume64
Issue number11
DOIs
StatePublished - Jan 1 2000

Fingerprint

Persea
Macrophages
Nitric Oxide Synthase Type II
Prostaglandin-Endoperoxide Synthases
Nitric Oxide Synthase
Hydrogen Peroxide
Skin
Tetradecanoylphorbol Acetate
Cyclooxygenase 2
Interferons
Lipopolysaccharides
Acetates
Cells
persenone A
Inflammation
Neoplasms
Proteins

Keywords

  • Cyclooxygenase-2
  • Hydrogen peroxide
  • Inducible nitric oxide synthase
  • Persenone A

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

An avocado constituent, persenone A, suppresses expression of inducible forms of nitric oxide synthase and cyclooxygenase in macrophages, and hydrogen peroxide generation in mouse skin. / Kim, Oe Kyung; Murakami, Akira; Takahashi, Daisuke; Nakamura, Yoshimasa; Torikai, Koji; Kim, Ha Won; Ohigashi, Hajime.

In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 11, 01.01.2000, p. 2504-2507.

Research output: Contribution to journalArticle

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