An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy: Development, localization, and biosynthesis

F. Gonzalez-Fernandez, R. A. Landers, P. A. Glazebrook, S. L. Fong, Gregory I Liou, D. M. Lam, C. D. Bridges

Research output: Contribution to journalArticle

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Abstract

Interstitial retinol-binding protein (IRBP) is a soluble glycoprotein in the interphotoreceptor matrix of bovine, human, monkey, and rat eyes. It may transport retinol between the retinal pigment epithelium and the neural retina. In light-reared Royal College of Surgeons (RCS) and RCS retinal dystrophy gene (rdy)+ rats, the amount of IRBP in the interphotoreceptor matrix increased in corresponding proportion to the amount of total rhodopsin through postnatal day 22 (P22). In the RCS-rdy+ rats, the amount increased slightly after P23. However, in the RCS rats there was a rapid fall in the quantity of IRBP as the photoreceptors degenerated between P23 and P29. No IRBP was detected by immunocytochemistry in rats at P28. The amount of rhodopsin fell more slowly. Although retinas from young RCS and RCS-rdy+ rats were able to synthesize and secrete IRBP, this ability was lost in retinas from older RCS rats (P51, P88) but not their congenic controls. The photoreceptor cells have degenerated at these ages in the RCS animals, and may therefore be the retinal cells responsible for IRBP synthesis. The putative function of IRBP in the extracellular transport of retinoids during the visual cycle is consistent with a defect in retinol transport in the RCS rat reported by others.

Original languageEnglish (US)
Pages (from-to)2092-2098
Number of pages7
JournalJournal of Cell Biology
Volume99
Issue number6
DOIs
StatePublished - Dec 1 1984

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Retinal Dystrophies
Vitamin A
Glycoproteins
Retina
Rhodopsin
Genes
Surgeons
Photoreceptor Cells
Retinal Pigment Epithelium
Retinoids
interstitial retinol-binding protein
Haplorhini
Immunohistochemistry
Light

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gonzalez-Fernandez, F., Landers, R. A., Glazebrook, P. A., Fong, S. L., Liou, G. I., Lam, D. M., & Bridges, C. D. (1984). An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy: Development, localization, and biosynthesis. Journal of Cell Biology, 99(6), 2092-2098. https://doi.org/10.1083/jcb.99.6.2092

An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy : Development, localization, and biosynthesis. / Gonzalez-Fernandez, F.; Landers, R. A.; Glazebrook, P. A.; Fong, S. L.; Liou, Gregory I; Lam, D. M.; Bridges, C. D.

In: Journal of Cell Biology, Vol. 99, No. 6, 01.12.1984, p. 2092-2098.

Research output: Contribution to journalArticle

Gonzalez-Fernandez, F, Landers, RA, Glazebrook, PA, Fong, SL, Liou, GI, Lam, DM & Bridges, CD 1984, 'An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy: Development, localization, and biosynthesis', Journal of Cell Biology, vol. 99, no. 6, pp. 2092-2098. https://doi.org/10.1083/jcb.99.6.2092
Gonzalez-Fernandez, F. ; Landers, R. A. ; Glazebrook, P. A. ; Fong, S. L. ; Liou, Gregory I ; Lam, D. M. ; Bridges, C. D. / An extracellular retinol-binding glycoprotein in the eyes of mutant rats with retinal dystrophy : Development, localization, and biosynthesis. In: Journal of Cell Biology. 1984 ; Vol. 99, No. 6. pp. 2092-2098.
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