An intrinsic membrane protein in oyster sperm stimulates spawning behaviors in Crassostrea virginica: Implications for aquaculture

Patrick Rice, Sammy M. Ray, Sherry J. Painter, Gregg Thomas Nagle

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pheromones are thought to play a critical role in triggering spawning in oysters, but none have been identified to date. Male and female oysters are stimulated to spawn by oyster sperm. Male oysters are also stimulated to spawn by oyster eggs, but females are not. Earlier studies suggest that the spawning activity associated with sperm may be membrane-bound. As a first step toward isolating and characterizing a spawning pheromone in sperm of the oyster Crassostrea virginica, we used: (1) an extraction and purification procedure for preparing oyster sperm membranes; (2) a method to selectively remove extrinsic sperm membrane-associated proteins; and (3) a bioassay to monitor the robust, repetitive adductor muscle contractions that occur concurrently with oyster spawning. This report presents evidence that the candidate oyster sperm pheromone is a heat- and trypsin-sensitive intrinsic membrane protein. A synthetic or recombinant fragment of the pheromone could be used in the aquaculture industry to induce spawning in oysters.

Original languageEnglish (US)
Pages (from-to)715-718
Number of pages4
JournalJournal of Shellfish Research
Volume21
Issue number2
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Crassostrea virginica
membrane proteins
sperm
oysters
aquaculture
spawning
spermatozoa
membrane
pheromone
protein
pheromones
aquaculture industry
contraction
purification
bioassay
muscle
egg
muscle contraction
trypsin
bioassays

Keywords

  • Bivalve
  • Crassostrea virginica
  • Mollusk
  • Oyster
  • Pheromone
  • Sperm

ASJC Scopus subject areas

  • Aquatic Science

Cite this

An intrinsic membrane protein in oyster sperm stimulates spawning behaviors in Crassostrea virginica : Implications for aquaculture. / Rice, Patrick; Ray, Sammy M.; Painter, Sherry J.; Nagle, Gregg Thomas.

In: Journal of Shellfish Research, Vol. 21, No. 2, 01.12.2002, p. 715-718.

Research output: Contribution to journalArticle

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