TY - JOUR
T1 - Analyses of autoantigens in a new form of endemic pemphigus foliaceus in Colombia
AU - Abrèu-Velez, Ana María
AU - Beutner, Ernst H.
AU - Motoya, Fernando
AU - Bollag, Wendy B.
AU - Hashimoto, Takashi
N1 - Funding Information:
Funding sources: Grants from the Embassy of Japan in Colombia; Mineros de Antioquia, S.A.; the Universidad de Antioquia; Medical College of Georgia; and the National Institutes of Health (grant no. AR45212). Dr Abrèu-Velez received a scholarship from Colciencias, Colombia. Studies at Beutner Laboratories on monkey esophagus sections were aided by Beutner Laboratories with National Institutes of Health grant no. RR00163 to the Oregon Primate Center.
Funding Information:
We extend our thanks to the people of El Bagre and to all public and private companies in Colombia that supported our program. We also acknowledge our co-workers who participated in the visits to El Bagre for their contribution to this research and humanitarian program. We gratefully appreciate Francois Tron, MD, Joly Pascal, MD, Fabianne Jouen-Beades, MD, Danielle Gilbert, PhD, and Vincent Saulot, PhD, Institut National de la Santé et de la Recherche Médicale (INSERM), France, for their kind encouragement and for generously providing us with the MCF-12 cells. In addition, we thank Rhea-Beth Markowitz, PhD, of the Medical College of Georgia for her excellent editorial assistance. This article represents a portion of the PhD thesis of Ana María Abrèu-Velez, who received a scholarship from Colciencias, Colombia, administered by the Latin American Scholarship Program of American Universities (USA).
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Background: We previously described a new focus of endemic pemphigus foliaceus in rural areas of El Bagre, Colombia, with clinical and direct immunofluorescence characteristics of pemphigus erythematosus. Objective: The aim of this study was to characterize autoantigen profiles for 34 serum samples obtained from patients with this condition. Methods: Immunofluorescence, various immunoblot analyses with different antigen sources and detection methods, and immunoprecipitation were performed. Results: Immunofluorescence with the use of human skin sections showed IgG autoantibodies against keratinocyte cell surfaces in all 34 serum samples. Some samples also showed weak reactivity with the basement membrane zone, The results of immunoblot and immunoprecipitation analysis indicated that all sera had antibodies reactive with desmoglein 1, the pemphigus foliaceus antigen. In addition, in various immunoblot assays, many sera reacted with several other proteins with molecular weights of 250 kd, 210 kd, and 190 kd, which appear to correspond to desmoplakin I, envoplakin, and periplakin, respectively. Conclusion: This endemic pemphigus disease in El Bagre showed immunologic features similar to pemphigus foliaceus or erythematosus. In addition, paraneoplastic pemphigus-like reactivity with various epidermal antigens was detected.
AB - Background: We previously described a new focus of endemic pemphigus foliaceus in rural areas of El Bagre, Colombia, with clinical and direct immunofluorescence characteristics of pemphigus erythematosus. Objective: The aim of this study was to characterize autoantigen profiles for 34 serum samples obtained from patients with this condition. Methods: Immunofluorescence, various immunoblot analyses with different antigen sources and detection methods, and immunoprecipitation were performed. Results: Immunofluorescence with the use of human skin sections showed IgG autoantibodies against keratinocyte cell surfaces in all 34 serum samples. Some samples also showed weak reactivity with the basement membrane zone, The results of immunoblot and immunoprecipitation analysis indicated that all sera had antibodies reactive with desmoglein 1, the pemphigus foliaceus antigen. In addition, in various immunoblot assays, many sera reacted with several other proteins with molecular weights of 250 kd, 210 kd, and 190 kd, which appear to correspond to desmoplakin I, envoplakin, and periplakin, respectively. Conclusion: This endemic pemphigus disease in El Bagre showed immunologic features similar to pemphigus foliaceus or erythematosus. In addition, paraneoplastic pemphigus-like reactivity with various epidermal antigens was detected.
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U2 - 10.1067/S0190-9622(03)00852-1
DO - 10.1067/S0190-9622(03)00852-1
M3 - Article
C2 - 14512904
AN - SCOPUS:18244430088
VL - 49
SP - 609
EP - 614
JO - Journal of the American Academy of Dermatology
JF - Journal of the American Academy of Dermatology
SN - 0190-9622
IS - 4
ER -