Fast, reproducible, and sensitive methods for the extraction and analysis of aflatoxin B1 in human liver, blood, and urine by high-pressure liquid chromatography are described. Recovery of aflatoxin B1 from spiked mouse liver homogenates was quantitative (>97%) over a range of 1.6-1600 ng/g. Better than 79% recovery of aflatoxin B1 was obtained from human blood samples spiked with 1-50 ng/ml. Using these procedures, liver samples from patients with primary hepatocellular carcinoma and liver, blood, and urine from patients diagnosed with Reye's syndrome were analyzed for aflatoxin B1. Aflatoxin B1 was detected in 4 of the 6 liver samples from primary hepatocellular carcinoma patients. Aflatoxin B1 also was detected in 11 of 14 liver samples, 4 of 6 blood samples, and 4 of 5 urine samples from patients with Reye's syndrome.
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