Analysis of cholesteryl ester transfer activity in adipose tissue

G. X. Shen, J. Y. Zhang, R. Blanchard, H. F. Zhang, M. Hayden, R. McPherson, A. Angel

Research output: Contribution to journalArticle

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Abstract

Transfer of cholesteryl ester (CE) between lipoproteins is mediated by a plasma glycoprotein called cholesteryl ester transfer protein (CETP) which plays an important role in reverse cholesterol transport between peripheral tissues and the liver. Adipose tissue is a major site of cholesterol storage and lipoprotein interactions. CE transfer activity in cultured adipose tissue from several species was analyzed by using a CE transfer assay in vitro. Significant amounts of CE transfer activity were detected in conditioned media of cultured adipose tissues from human, hamster and transgenic mice expressing CETP. The amount of CE transfer activity released from cultured adipose tissue varied among species and adipose regions. Monoclonal antibodies against human CETP (subclone TP2) inhibited CE transfer activity released from cultured adipose tissue of hamsters and CETP transgenic mice. CE transfer activity was also assayed in the aqueous phase of adipose tissue homogenates. Accumulation of CE transfer activity in the medium was associated with a reciprocal decrease in adipose tissue activity consistent with the secretion of CE transfer activity from a labile pool. The levels of CE transfer activity positively correlated to the amount of CETP mass in the conditioned medium and the aqueous phase of tissue homogenates from cultured hamster adipose tissue. The results of the present study indicate that the measurement of CE transfer activity in adipose tissue reliably characterizes the secretion and storage of CETP in adipose tissue.

Original languageEnglish (US)
JournalInternational Journal of Obesity
Volume20
Issue numberSUPPL. 3
StatePublished - Apr 22 1996

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Cholesterol Esters
Adipose Tissue
Cholesterol Ester Transfer Proteins
Cricetinae
Conditioned Culture Medium
Transgenic Mice
Lipoproteins
Glycoproteins
Cholesterol
Monoclonal Antibodies

Keywords

  • Adipose tissue
  • Cholesteryl ester transfer activity

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Shen, G. X., Zhang, J. Y., Blanchard, R., Zhang, H. F., Hayden, M., McPherson, R., & Angel, A. (1996). Analysis of cholesteryl ester transfer activity in adipose tissue. International Journal of Obesity, 20(SUPPL. 3).

Analysis of cholesteryl ester transfer activity in adipose tissue. / Shen, G. X.; Zhang, J. Y.; Blanchard, R.; Zhang, H. F.; Hayden, M.; McPherson, R.; Angel, A.

In: International Journal of Obesity, Vol. 20, No. SUPPL. 3, 22.04.1996.

Research output: Contribution to journalArticle

Shen, GX, Zhang, JY, Blanchard, R, Zhang, HF, Hayden, M, McPherson, R & Angel, A 1996, 'Analysis of cholesteryl ester transfer activity in adipose tissue', International Journal of Obesity, vol. 20, no. SUPPL. 3.
Shen GX, Zhang JY, Blanchard R, Zhang HF, Hayden M, McPherson R et al. Analysis of cholesteryl ester transfer activity in adipose tissue. International Journal of Obesity. 1996 Apr 22;20(SUPPL. 3).
Shen, G. X. ; Zhang, J. Y. ; Blanchard, R. ; Zhang, H. F. ; Hayden, M. ; McPherson, R. ; Angel, A. / Analysis of cholesteryl ester transfer activity in adipose tissue. In: International Journal of Obesity. 1996 ; Vol. 20, No. SUPPL. 3.
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