Analysis of GNAS mutations in cemento-ossifying fibromas and cemento-osseous dysplasias of the jaws

Milan M. Patel, Jonathan F. Wilkey, Rafik A Abdelsayed, Nisha J. D'Silva, Carl Malchoff, Sanjay M. Mallya

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objectives: It is well established that fibrous dysplasia (FD) is caused by mutations of the Arg201 codon of the GNAS gene. However, the role of GNAS mutation in the pathogenesis of cement-osseous dysplasias (COD) and cemento-ossifying fibromas (COF) is not fully known. In this study, we examined COD and COF for mutations at the Arg201 codon of the GNAS gene. Study design: The study sample included formalin-fixed, paraffin-embedded tissue blocks from 8 COF and 24 COD. We used 2 PCR-RFLP methods to detect mutations at the Arg201 codon of the GNAS gene. Results: Mutations at the Arg201 codon of the GNAS gene were not present in any of the COD and COF examined. Conclusions: GNAS mutations do not play a role in the pathogenesis of COD and COF. This highlights a clear molecular distinction between FD and other histologically similar fibro-osseous lesions of the jaws.

Original languageEnglish (US)
Pages (from-to)739-743
Number of pages5
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
Volume109
Issue number5
DOIs
StatePublished - May 1 2010

Fingerprint

Ossifying Fibroma
Jaw
Codon
Mutation
Genes
Restriction Fragment Length Polymorphisms
Paraffin
Formaldehyde
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Analysis of GNAS mutations in cemento-ossifying fibromas and cemento-osseous dysplasias of the jaws. / Patel, Milan M.; Wilkey, Jonathan F.; Abdelsayed, Rafik A; D'Silva, Nisha J.; Malchoff, Carl; Mallya, Sanjay M.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, Vol. 109, No. 5, 01.05.2010, p. 739-743.

Research output: Contribution to journalArticle

Patel, Milan M. ; Wilkey, Jonathan F. ; Abdelsayed, Rafik A ; D'Silva, Nisha J. ; Malchoff, Carl ; Mallya, Sanjay M. / Analysis of GNAS mutations in cemento-ossifying fibromas and cemento-osseous dysplasias of the jaws. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. 2010 ; Vol. 109, No. 5. pp. 739-743.
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AU - Malchoff, Carl

AU - Mallya, Sanjay M.

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AB - Objectives: It is well established that fibrous dysplasia (FD) is caused by mutations of the Arg201 codon of the GNAS gene. However, the role of GNAS mutation in the pathogenesis of cement-osseous dysplasias (COD) and cemento-ossifying fibromas (COF) is not fully known. In this study, we examined COD and COF for mutations at the Arg201 codon of the GNAS gene. Study design: The study sample included formalin-fixed, paraffin-embedded tissue blocks from 8 COF and 24 COD. We used 2 PCR-RFLP methods to detect mutations at the Arg201 codon of the GNAS gene. Results: Mutations at the Arg201 codon of the GNAS gene were not present in any of the COD and COF examined. Conclusions: GNAS mutations do not play a role in the pathogenesis of COD and COF. This highlights a clear molecular distinction between FD and other histologically similar fibro-osseous lesions of the jaws.

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