Analysis of TI-6Al-4V Implants Place with Fibroblast Growth Factor 1 in Rat Tibiae

Michael Scott McCracken, Jack E. Lemons, Kurt Zinn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Titanium-aluminum-vanadium (Ti-6Al-4V) implants were placed in the tibiae of 32 rats (male Sprague-Dawley, 350 g) to examine healing and bone response. Half of the implants were treated with fibroblast growth factor 1 (FGF-1) delivered in an activated fibrinogen matrix. Animals were injected with a radiopharmaceutical imaging agent, technetium-99m-methylene diphosphonate (Tc-99m-MDP), which concentrates in bone, especially in areas of higher osteoblastic activity. Binding of Tc-99m-MDP to the implant was detected in vivo by Anger gamma camera imaging. Fourteen days after implant surgery, specimens were recovered and prepared for histomorphometric analysis. Histologic examination revealed that samples treated with FGF-1 demonstrated significantly greater amounts of bone-to-implant contact (P < .05) compared to controls. Also, FGF-1-treated samples showed significantly greater amounts of bone (percent volume) adjacent to implants (P < .005). These findings were supported by analyses of the non-invasive Tc-99m-MDP images, which demonstrated significantly greater uptake of Tc-99m-MDP adjacent to FGF-1-treated implants (P < .05). Results of the experiments supported the hypothesis that FGF-1 could increase bone production around implants in a rat model.

Original languageEnglish (US)
Pages (from-to)495-502
Number of pages8
JournalInternational Journal of Oral and Maxillofacial Implants
Volume16
Issue number4
StatePublished - Dec 1 2001

Fingerprint

Fibroblast Growth Factor 1
Technetium Tc 99m Medronate
Tibia
Technetium
Bone and Bones
Vanadium
Radiopharmaceuticals
Anger
Titanium
Aluminum
Radionuclide Imaging
Fibrinogen
Sprague Dawley Rats

Keywords

  • Dental implants
  • Fibroblast growth factor
  • Osseointegration
  • Radioimmunodetection

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Analysis of TI-6Al-4V Implants Place with Fibroblast Growth Factor 1 in Rat Tibiae. / McCracken, Michael Scott; Lemons, Jack E.; Zinn, Kurt.

In: International Journal of Oral and Maxillofacial Implants, Vol. 16, No. 4, 01.12.2001, p. 495-502.

Research output: Contribution to journalArticle

McCracken, Michael Scott ; Lemons, Jack E. ; Zinn, Kurt. / Analysis of TI-6Al-4V Implants Place with Fibroblast Growth Factor 1 in Rat Tibiae. In: International Journal of Oral and Maxillofacial Implants. 2001 ; Vol. 16, No. 4. pp. 495-502.
@article{c46967b5580f4a068bf3b711a4006ec1,
title = "Analysis of TI-6Al-4V Implants Place with Fibroblast Growth Factor 1 in Rat Tibiae",
abstract = "Titanium-aluminum-vanadium (Ti-6Al-4V) implants were placed in the tibiae of 32 rats (male Sprague-Dawley, 350 g) to examine healing and bone response. Half of the implants were treated with fibroblast growth factor 1 (FGF-1) delivered in an activated fibrinogen matrix. Animals were injected with a radiopharmaceutical imaging agent, technetium-99m-methylene diphosphonate (Tc-99m-MDP), which concentrates in bone, especially in areas of higher osteoblastic activity. Binding of Tc-99m-MDP to the implant was detected in vivo by Anger gamma camera imaging. Fourteen days after implant surgery, specimens were recovered and prepared for histomorphometric analysis. Histologic examination revealed that samples treated with FGF-1 demonstrated significantly greater amounts of bone-to-implant contact (P < .05) compared to controls. Also, FGF-1-treated samples showed significantly greater amounts of bone (percent volume) adjacent to implants (P < .005). These findings were supported by analyses of the non-invasive Tc-99m-MDP images, which demonstrated significantly greater uptake of Tc-99m-MDP adjacent to FGF-1-treated implants (P < .05). Results of the experiments supported the hypothesis that FGF-1 could increase bone production around implants in a rat model.",
keywords = "Dental implants, Fibroblast growth factor, Osseointegration, Radioimmunodetection",
author = "McCracken, {Michael Scott} and Lemons, {Jack E.} and Kurt Zinn",
year = "2001",
month = "12",
day = "1",
language = "English (US)",
volume = "16",
pages = "495--502",
journal = "The International journal of oral & maxillofacial implants",
issn = "0882-2786",
publisher = "Quintessence Publishing Company",
number = "4",

}

TY - JOUR

T1 - Analysis of TI-6Al-4V Implants Place with Fibroblast Growth Factor 1 in Rat Tibiae

AU - McCracken, Michael Scott

AU - Lemons, Jack E.

AU - Zinn, Kurt

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Titanium-aluminum-vanadium (Ti-6Al-4V) implants were placed in the tibiae of 32 rats (male Sprague-Dawley, 350 g) to examine healing and bone response. Half of the implants were treated with fibroblast growth factor 1 (FGF-1) delivered in an activated fibrinogen matrix. Animals were injected with a radiopharmaceutical imaging agent, technetium-99m-methylene diphosphonate (Tc-99m-MDP), which concentrates in bone, especially in areas of higher osteoblastic activity. Binding of Tc-99m-MDP to the implant was detected in vivo by Anger gamma camera imaging. Fourteen days after implant surgery, specimens were recovered and prepared for histomorphometric analysis. Histologic examination revealed that samples treated with FGF-1 demonstrated significantly greater amounts of bone-to-implant contact (P < .05) compared to controls. Also, FGF-1-treated samples showed significantly greater amounts of bone (percent volume) adjacent to implants (P < .005). These findings were supported by analyses of the non-invasive Tc-99m-MDP images, which demonstrated significantly greater uptake of Tc-99m-MDP adjacent to FGF-1-treated implants (P < .05). Results of the experiments supported the hypothesis that FGF-1 could increase bone production around implants in a rat model.

AB - Titanium-aluminum-vanadium (Ti-6Al-4V) implants were placed in the tibiae of 32 rats (male Sprague-Dawley, 350 g) to examine healing and bone response. Half of the implants were treated with fibroblast growth factor 1 (FGF-1) delivered in an activated fibrinogen matrix. Animals were injected with a radiopharmaceutical imaging agent, technetium-99m-methylene diphosphonate (Tc-99m-MDP), which concentrates in bone, especially in areas of higher osteoblastic activity. Binding of Tc-99m-MDP to the implant was detected in vivo by Anger gamma camera imaging. Fourteen days after implant surgery, specimens were recovered and prepared for histomorphometric analysis. Histologic examination revealed that samples treated with FGF-1 demonstrated significantly greater amounts of bone-to-implant contact (P < .05) compared to controls. Also, FGF-1-treated samples showed significantly greater amounts of bone (percent volume) adjacent to implants (P < .005). These findings were supported by analyses of the non-invasive Tc-99m-MDP images, which demonstrated significantly greater uptake of Tc-99m-MDP adjacent to FGF-1-treated implants (P < .05). Results of the experiments supported the hypothesis that FGF-1 could increase bone production around implants in a rat model.

KW - Dental implants

KW - Fibroblast growth factor

KW - Osseointegration

KW - Radioimmunodetection

UR - http://www.scopus.com/inward/record.url?scp=0035403996&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035403996&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 495

EP - 502

JO - The International journal of oral & maxillofacial implants

JF - The International journal of oral & maxillofacial implants

SN - 0882-2786

IS - 4

ER -