Anisomycin, an inhibitor of protein synthesis, blocks late phases of LTP phenomena in the hippocampal CA1 region in vitro

Uwe Frey, Manfred Krug, Klaus G. Reymann, Hansjuergen Matthies

Research output: Contribution to journalArticle

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Abstract

Long-term potentiation (LTP) with its extremely long duration has been frequently regarded as an elementary mechanism of information storage in the nervous system or at least as a suitable model for the study of mechanisms underlying functional plasticity and processes of learning and memory formation. Considering the necessity of an increased protein synthesis for memory consolidation and for the maintenance of LTP in granular synapses in vivo it was of interest to determine whether the LTP of the CA1 region of the hippocampus depends on protein synthesis as well. For the solution of this question anisomycin (ANI), a reversible blocker of protein synthesis, was used at a concentration of 20 μM, which blocked the [3H]leucine incorporation in hippocampal slices by at least 85%. It has been shown that in the CA1 region in vitro the maintenance of LTP (i.e. a late phase >5 h) depends on an ongoing protein synthesis. A 3-h treatment with ANI immediately following multiple tetanization resulted in gradually developing loss of field excitatory postsynaptic potential (EPSP) and population spike (PS) potentiation (15 ± 19% increase of the PS instead of the 96 ± 14% increase in non-treated control experiments at the 8th h after tetanization). Furthermore, a late PS potentiation (>6 h) of a second non-tetanized pathway to CA1 pyramidal cells has been observed (increase by 64 ± 18% at the 8th h) for the first time. This potentiation was ANI-sensitive as well and suggests that the maintenance of LTP is dependent on a postsynaptic mechanism.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalBrain Research
Volume452
Issue number1-2
DOIs
StatePublished - Jun 14 1988

Fingerprint

Anisomycin
Hippocampal CA1 Region
Protein Synthesis Inhibitors
Long-Term Potentiation
Maintenance
Proteins
Population
Pyramidal Cells
Excitatory Postsynaptic Potentials
Information Storage and Retrieval
Leucine
Synapses
Nervous System
Hippocampus
In Vitro Techniques
Learning

Keywords

  • Anisomycin
  • Field potential
  • Hippocampal CA1 in vitro
  • Long-term potentiation
  • Protein synthesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Anisomycin, an inhibitor of protein synthesis, blocks late phases of LTP phenomena in the hippocampal CA1 region in vitro. / Frey, Uwe; Krug, Manfred; Reymann, Klaus G.; Matthies, Hansjuergen.

In: Brain Research, Vol. 452, No. 1-2, 14.06.1988, p. 57-65.

Research output: Contribution to journalArticle

Frey, Uwe ; Krug, Manfred ; Reymann, Klaus G. ; Matthies, Hansjuergen. / Anisomycin, an inhibitor of protein synthesis, blocks late phases of LTP phenomena in the hippocampal CA1 region in vitro. In: Brain Research. 1988 ; Vol. 452, No. 1-2. pp. 57-65.
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