Anti-inflammatory influence of P-selectin on human mononuclear cells

Courtney L. Sloan, Gloria Sloan, Joseph Gerard Cannon

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The purpose of this study was to determine if P-selectin, an adhesion molecule involved in the transendothelial movement of leukocytes, might also have a direct influence on the function of cells that come into contact with it. Human peripheral blood mononuclear cells were incubated on immobilized P-selectin or a control substrate (bovine serum albumin, BSA) and stimulated with bacterial lipopolysaccharide (LPS). After 24 h, the concentrations of proinflammatory cytokines in the supernatants of LPS-stimulated cells incubated on P-selectin were < 50% of those produced by cells incubated on BSA (interleukin-1β : P = 0.001, tumor necrosis factor-α : P = 0.004, and interferon-γ : P = 0.026). In contrast, cells incubated on P-selectin produced 74% more of the anti-inflammatory cytokine interleukin-10 than cells incubated on BSA (P = 0.013). Neither P-selectin nor BSA stimulated cytokine production in the absence of LPS. Thus, P-selectin modulated the cytokine secretion of peripheral blood mononuclear cells in a coordinated manner that reduced the inflammatory potential of the cells.

Original languageEnglish (US)
Pages (from-to)166-169
Number of pages4
JournalVascular Pharmacology
Volume44
Issue number3
DOIs
StatePublished - Mar 1 2006

Keywords

  • Inflammation
  • Interleukin-1 β
  • Interleukin-10
  • P-selectin

ASJC Scopus subject areas

  • Pharmacology

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