Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract

Ravirajsinh N. Jadeja, Menaka Thounaojam, Umed V. Ramani, Ranjitsinh V. Devkar, A. V. Ramachandran

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Clerodendron glandulosum.Coleb leaf aqueous extract (CG) is traditionally used by people of North-East India to alleviate symptoms of diabetes, obesity and hypertension. Previous study from our laboratory have documented anti-diabetic and anti-hypertensive properties of CG extract but, till date there are no pharmacological studies available on its anti-obesity potential. This inventory investigates the underlining molecular mechanism/s of CG induced regulation of in vivo HFD induced obesity and in vitro adipocyte differentiation. Aim: To evaluate effects of CG extract on (i) expression of genes regulating visceral adiposity and (ii) in vitro adipocyte differentiation and LEP release. Materials and methods: Body weight, lee index, plasma lipids and LEP, mRNA expression of PPARγ-2, SREBP1c, FAS, CPT-1 and LEP in epididymal adipose tissue of control and experimental groups were evaluated. Also, potential of CG extract on in vitro adipocyte differentiation and LEP release was assessed. Results: Supplementation of CG extract to HFD fed mice significantly prevented HFD induced increment in bodyweight, lee index, plasma lipids and LEP, visceral adiposity and adipocyte hypertrophy. Also, CG extract supplementation resulted in down regulation of PPARγ-2, SREBP1c, FAS and LEP expression along with up-regulation of CPT-1 in epididymal adipose tissue compared to HFD fed mice. In vitro study recorded significant anti-adipogenic effect of CG extract that resulted in decreased adipogenesis, TG accumulation, LEP release, G3PDH activity along with higher glycerol release without significantly altering viability of 3T3L1 pre-adipocytes. Conclusions: Clerodendron glandulosum.Coleb extract prevents adipocyte differentiation and visceral adiposity by down regulation of PPARγ-2 related genes and Lep expression thus validating its traditional therapeutic use in controlling obesity.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalJournal of Ethnopharmacology
Volume135
Issue number2
DOIs
StatePublished - May 17 2011
Externally publishedYes

Fingerprint

Clerodendrum
Adipocytes
Obesity
Peroxisome Proliferator-Activated Receptors
Adiposity
Adipose Tissue
Down-Regulation
Lipids
Gene Expression
Adipogenesis
Therapeutic Uses
Glycerol
Hypertrophy
Antihypertensive Agents
India
Up-Regulation
Body Weight
Pharmacology
Hypertension
Equipment and Supplies

Keywords

  • 3T3L1 cells
  • Clerodendron glandulosum.Coleb
  • Leptin
  • Obesity
  • PPARγ

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract. / Jadeja, Ravirajsinh N.; Thounaojam, Menaka; Ramani, Umed V.; Devkar, Ranjitsinh V.; Ramachandran, A. V.

In: Journal of Ethnopharmacology, Vol. 135, No. 2, 17.05.2011, p. 338-343.

Research output: Contribution to journalArticle

Jadeja, Ravirajsinh N. ; Thounaojam, Menaka ; Ramani, Umed V. ; Devkar, Ranjitsinh V. ; Ramachandran, A. V. / Anti-obesity potential of Clerodendron glandulosum.Coleb leaf aqueous extract. In: Journal of Ethnopharmacology. 2011 ; Vol. 135, No. 2. pp. 338-343.
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