Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation

Walter G. Renné, Amanda Lindner, Anthony S. Mennito, Kelli A. Agee, David Henry Pashley, Daniel Willett, David Sentelle, Michael Defee, Michael Schmidt, Camila Sabatini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: This study investigated the antibacterial properties and micro-hardness of polyacrylic acid (PAA)-coated copper iodide (CuI) nanoparticles incorporated into glass ionomer-based materials, and the effect of PAA-CuI on collagen degradation. Materials and methods: PAA-CuI nanoparticles were incorporated into glass ionomer (GI), Ionofil Molar AC, and resin-modified glass ionomer (RMGI), Vitrebond, at 0.263 wt%. The antibacterial properties against Streptococcus mutans (n = 6/group) and surface micro-hardness (n = 5/group) were evaluated. Twenty dentin beams were completely demineralized in 10 wt% phosphoric acid and equally divided in two groups (n = 10/group) for incubation in simulated body fluid (SBF) or SBF containing 1 mg/ml PAA-CuI. The amount of dry mass loss and hydroxyproline (HYP) released were quantified. Kruskal-Wallis, Student’s t test, two-way ANOVA, and Mann-Whitney were used to analyze the antibacterial, micro-hardness, dry mass, and HYP release data, respectively (p < 0.05). Results: Addition of PAA-CuI nanoparticles into the glass ionomer matrix yielded significant reduction (99.999 %) in the concentration of bacteria relative to the control groups. While micro-hardness values of PAA-CuI-doped GI were no different from its control, PAA-CuI-doped RMGI demonstrated significantly higher values than its control. A significant decrease in dry mass weight was shown only for the control beams (10.53 %, p = 0.04). Significantly less HYP was released from beams incubated in PAA-CuI relative to the control beams (p < 0.001). Conclusions: PAA-CuI nanoparticles are an effective additive to glass ionomer-based materials as they greatly enhance their antibacterial properties and reduce collagen degradation without an adverse effect on their mechanical properties. Clinical relevance: The use of copper-doped glass ionomer-based materials under composite restorations may contribute to an increased longevity of adhesive restorations, because of their enhanced antibacterial properties and reduced collagen degradation.

Original languageEnglish (US)
Pages (from-to)369-379
Number of pages11
JournalClinical oral investigations
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2017

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carbopol 940
Iodides
Nanoparticles
Copper
Collagen
Hardness
Hydroxyproline
Body Fluids
glass ionomer
Streptococcus mutans
Dentin

Keywords

  • Antibacterial
  • Collagen degradation
  • Glass ionomer
  • Matrix metalloproteinases
  • Micro-hardness

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation. / Renné, Walter G.; Lindner, Amanda; Mennito, Anthony S.; Agee, Kelli A.; Pashley, David Henry; Willett, Daniel; Sentelle, David; Defee, Michael; Schmidt, Michael; Sabatini, Camila.

In: Clinical oral investigations, Vol. 21, No. 1, 01.01.2017, p. 369-379.

Research output: Contribution to journalArticle

Renné, WG, Lindner, A, Mennito, AS, Agee, KA, Pashley, DH, Willett, D, Sentelle, D, Defee, M, Schmidt, M & Sabatini, C 2017, 'Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation', Clinical oral investigations, vol. 21, no. 1, pp. 369-379. https://doi.org/10.1007/s00784-016-1799-y
Renné, Walter G. ; Lindner, Amanda ; Mennito, Anthony S. ; Agee, Kelli A. ; Pashley, David Henry ; Willett, Daniel ; Sentelle, David ; Defee, Michael ; Schmidt, Michael ; Sabatini, Camila. / Antibacterial properties of copper iodide-doped glass ionomer-based materials and effect of copper iodide nanoparticles on collagen degradation. In: Clinical oral investigations. 2017 ; Vol. 21, No. 1. pp. 369-379.
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AU - Lindner, Amanda

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AU - Agee, Kelli A.

AU - Pashley, David Henry

AU - Willett, Daniel

AU - Sentelle, David

AU - Defee, Michael

AU - Schmidt, Michael

AU - Sabatini, Camila

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N2 - Objectives: This study investigated the antibacterial properties and micro-hardness of polyacrylic acid (PAA)-coated copper iodide (CuI) nanoparticles incorporated into glass ionomer-based materials, and the effect of PAA-CuI on collagen degradation. Materials and methods: PAA-CuI nanoparticles were incorporated into glass ionomer (GI), Ionofil Molar AC, and resin-modified glass ionomer (RMGI), Vitrebond, at 0.263 wt%. The antibacterial properties against Streptococcus mutans (n = 6/group) and surface micro-hardness (n = 5/group) were evaluated. Twenty dentin beams were completely demineralized in 10 wt% phosphoric acid and equally divided in two groups (n = 10/group) for incubation in simulated body fluid (SBF) or SBF containing 1 mg/ml PAA-CuI. The amount of dry mass loss and hydroxyproline (HYP) released were quantified. Kruskal-Wallis, Student’s t test, two-way ANOVA, and Mann-Whitney were used to analyze the antibacterial, micro-hardness, dry mass, and HYP release data, respectively (p < 0.05). Results: Addition of PAA-CuI nanoparticles into the glass ionomer matrix yielded significant reduction (99.999 %) in the concentration of bacteria relative to the control groups. While micro-hardness values of PAA-CuI-doped GI were no different from its control, PAA-CuI-doped RMGI demonstrated significantly higher values than its control. A significant decrease in dry mass weight was shown only for the control beams (10.53 %, p = 0.04). Significantly less HYP was released from beams incubated in PAA-CuI relative to the control beams (p < 0.001). Conclusions: PAA-CuI nanoparticles are an effective additive to glass ionomer-based materials as they greatly enhance their antibacterial properties and reduce collagen degradation without an adverse effect on their mechanical properties. Clinical relevance: The use of copper-doped glass ionomer-based materials under composite restorations may contribute to an increased longevity of adhesive restorations, because of their enhanced antibacterial properties and reduced collagen degradation.

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