Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

Levi H Makala, Y. Nishikawa, T. Kamada, X. Xuan, H. Nagasawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.

Original languageEnglish (US)
Pages (from-to)104-112
Number of pages9
JournalPathobiology
Volume69
Issue number2
DOIs
StatePublished - Dec 1 2001

Fingerprint

Macrophages
Peritoneal Cavity
Toxoplasma
Antigen Presentation
Peritoneal Macrophages
Dendritic Cells
Antigens
Histocompatibility Antigens Class II
Granulocyte-Macrophage Colony-Stimulating Factor
Major Histocompatibility Complex
Interleukin-4
Interferon-gamma
T-cells
Lipopolysaccharides
Intercellular Signaling Peptides and Proteins
Accessories
Spleen
T-Lymphocytes

Keywords

  • Antigen presentation
  • Mouse
  • Peritoneal macrophage-derived dendritic cell
  • Toxoplasma lysate antigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Makala, L. H., Nishikawa, Y., Kamada, T., Xuan, X., & Nagasawa, H. (2001). Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells. Pathobiology, 69(2), 104-112. https://doi.org/10.1159/000048763

Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells. / Makala, Levi H; Nishikawa, Y.; Kamada, T.; Xuan, X.; Nagasawa, H.

In: Pathobiology, Vol. 69, No. 2, 01.12.2001, p. 104-112.

Research output: Contribution to journalArticle

Makala, LH, Nishikawa, Y, Kamada, T, Xuan, X & Nagasawa, H 2001, 'Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells', Pathobiology, vol. 69, no. 2, pp. 104-112. https://doi.org/10.1159/000048763
Makala, Levi H ; Nishikawa, Y. ; Kamada, T. ; Xuan, X. ; Nagasawa, H. / Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells. In: Pathobiology. 2001 ; Vol. 69, No. 2. pp. 104-112.
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