Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells

L. H.C. Makala, Y. Nishikawa, T. Kamada, X. Xuan, H. Nagasawa

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Murine peritoneal cavity macrophage derived dendritic cells (PEC-DC) generated using early growth factors, interleukin 4 and granulocyte-macrophage colony-stimulating factor followed by maturation in interferon-gamma plus either, Toxoplasma lysate antigen (TLA) or lipopolysaccharide, bind TLA by a nonspecific mechanism and continue to express major histocompatibility complex class II antigens after 24 h of culture in vitro. Moreover, the proliferation of CD3+ spleen T cells from mice immunized with Toxoplasma gondii homogenate, induced by PEC-DC-mediated antigen presentation was statistically significant and of consistent amplitude. This accessory function of PEC-DC is antigen specific.

Original languageEnglish (US)
Pages (from-to)104-112
Number of pages9
JournalPathobiology
Volume69
Issue number2
DOIs
StatePublished - Dec 1 2001

Keywords

  • Antigen presentation
  • Mouse
  • Peritoneal macrophage-derived dendritic cell
  • Toxoplasma lysate antigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Makala, L. H. C., Nishikawa, Y., Kamada, T., Xuan, X., & Nagasawa, H. (2001). Antigen presentation by murine peritoneal cavity macrophage-derived dendritic cells. Pathobiology, 69(2), 104-112. https://doi.org/10.1159/000048763