Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells

Rupali R. Hire, Shalini Srivastava, Melissa B Davis, Ananda Kumar Konreddy, Dulal Panda

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Crocin, a component of saffron spice, is known to have an anticancer activity. However, the targets of crocin are not known. In this study, crocin was found to inhibit the proliferation of HCC70, HCC1806, HeLa and CCD1059sk cells by targeting microtubules. Crocin depolymerized both the interphase and mitotic microtubules of different cancer cells, inhibited mitosis and induced multipolar spindle formation in these cells. In vitro, crocin inhibited the assembly of pure tubulin as well as the assembly of microtubule-associated protein rich tubulin. Electron microscopic analysis showed that crocin inhibited microtubule assembly while it induced aggregation of tubulin at higher concentrations. Crocin co-eluted with tubulin suggesting that it binds to tubulin. Vinblastine inhibited the binding of crocin to tubulin while podophyllotoxin did not inhibit the crocin binding indicating that crocin binds at the vinblastine site on tubulin. The results suggested that crocin inhibited cell proliferation mainly by disrupting the microtubule network.

Original languageEnglish (US)
Article number44984
JournalScientific Reports
Volume7
DOIs
StatePublished - Mar 24 2017
Externally publishedYes

Fingerprint

Microtubules
Breast Neoplasms
Tubulin
Vinblastine
crocin
Podophyllotoxin
Spices
Microtubule-Associated Proteins
Interphase
HeLa Cells
Mitosis
Cell Proliferation
Electrons

ASJC Scopus subject areas

  • General

Cite this

Hire, R. R., Srivastava, S., Davis, M. B., Kumar Konreddy, A., & Panda, D. (2017). Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells. Scientific Reports, 7, [44984]. https://doi.org/10.1038/srep44984

Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells. / Hire, Rupali R.; Srivastava, Shalini; Davis, Melissa B; Kumar Konreddy, Ananda; Panda, Dulal.

In: Scientific Reports, Vol. 7, 44984, 24.03.2017.

Research output: Contribution to journalArticle

Hire, Rupali R. ; Srivastava, Shalini ; Davis, Melissa B ; Kumar Konreddy, Ananda ; Panda, Dulal. / Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells. In: Scientific Reports. 2017 ; Vol. 7.
@article{fb506a4593044794bce8d018a4f61790,
title = "Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells",
abstract = "Crocin, a component of saffron spice, is known to have an anticancer activity. However, the targets of crocin are not known. In this study, crocin was found to inhibit the proliferation of HCC70, HCC1806, HeLa and CCD1059sk cells by targeting microtubules. Crocin depolymerized both the interphase and mitotic microtubules of different cancer cells, inhibited mitosis and induced multipolar spindle formation in these cells. In vitro, crocin inhibited the assembly of pure tubulin as well as the assembly of microtubule-associated protein rich tubulin. Electron microscopic analysis showed that crocin inhibited microtubule assembly while it induced aggregation of tubulin at higher concentrations. Crocin co-eluted with tubulin suggesting that it binds to tubulin. Vinblastine inhibited the binding of crocin to tubulin while podophyllotoxin did not inhibit the crocin binding indicating that crocin binds at the vinblastine site on tubulin. The results suggested that crocin inhibited cell proliferation mainly by disrupting the microtubule network.",
author = "Hire, {Rupali R.} and Shalini Srivastava and Davis, {Melissa B} and {Kumar Konreddy}, Ananda and Dulal Panda",
year = "2017",
month = "3",
day = "24",
doi = "10.1038/srep44984",
language = "English (US)",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Antiproliferative Activity of Crocin Involves Targeting of Microtubules in Breast Cancer Cells

AU - Hire, Rupali R.

AU - Srivastava, Shalini

AU - Davis, Melissa B

AU - Kumar Konreddy, Ananda

AU - Panda, Dulal

PY - 2017/3/24

Y1 - 2017/3/24

N2 - Crocin, a component of saffron spice, is known to have an anticancer activity. However, the targets of crocin are not known. In this study, crocin was found to inhibit the proliferation of HCC70, HCC1806, HeLa and CCD1059sk cells by targeting microtubules. Crocin depolymerized both the interphase and mitotic microtubules of different cancer cells, inhibited mitosis and induced multipolar spindle formation in these cells. In vitro, crocin inhibited the assembly of pure tubulin as well as the assembly of microtubule-associated protein rich tubulin. Electron microscopic analysis showed that crocin inhibited microtubule assembly while it induced aggregation of tubulin at higher concentrations. Crocin co-eluted with tubulin suggesting that it binds to tubulin. Vinblastine inhibited the binding of crocin to tubulin while podophyllotoxin did not inhibit the crocin binding indicating that crocin binds at the vinblastine site on tubulin. The results suggested that crocin inhibited cell proliferation mainly by disrupting the microtubule network.

AB - Crocin, a component of saffron spice, is known to have an anticancer activity. However, the targets of crocin are not known. In this study, crocin was found to inhibit the proliferation of HCC70, HCC1806, HeLa and CCD1059sk cells by targeting microtubules. Crocin depolymerized both the interphase and mitotic microtubules of different cancer cells, inhibited mitosis and induced multipolar spindle formation in these cells. In vitro, crocin inhibited the assembly of pure tubulin as well as the assembly of microtubule-associated protein rich tubulin. Electron microscopic analysis showed that crocin inhibited microtubule assembly while it induced aggregation of tubulin at higher concentrations. Crocin co-eluted with tubulin suggesting that it binds to tubulin. Vinblastine inhibited the binding of crocin to tubulin while podophyllotoxin did not inhibit the crocin binding indicating that crocin binds at the vinblastine site on tubulin. The results suggested that crocin inhibited cell proliferation mainly by disrupting the microtubule network.

UR - http://www.scopus.com/inward/record.url?scp=85016137598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85016137598&partnerID=8YFLogxK

U2 - 10.1038/srep44984

DO - 10.1038/srep44984

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 44984

ER -