Apoptosis signaling pathway in T cells is composed of ICE/Ced-3 family proteases and MAP kinase kinase 6b

Shuang Huang, Yong Jiang, Zhuangjie Li, Eisuke Nishida, Patricia Mathias, Shengcai Lin, Richard J. Ulevitch, Glen R. Nemerow, Jiahuai Han

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

Fas/APO-1(CD95) ligation activates programmed cell death, a cellular process that plays an important role in the maturation of the host immune response. We show that activation of a specific MAP kinase kinase (MKK), MKK6b, is necessary and sufficient for Fas-induced apoptosis of Jurkat T cells. MKK6b activation occurs downstream of an interleukin-1 converting enzyme-like (ICE-like) protease(s), while execution of the apoptotic pathway by MKK6b requires both ICE- and CPP32-like proteases. Surprisingly, the p38 MAP kinase protein, a known substrate of MKK6b, does not participate in Fas/MKK6b-mediated apoptosis. These findings indicate a divergence of the MKK6b signaling pathways, one of which activates p38 and leads to regulation of gene expression, and one of which activates the ICE/Cad-3 family of proteases and leads to cell death. These studios represent a demonstration of an apoptotic pathway that is comprised of both the ICF/Ced-3 family of proteases and MAP kinase kinase 6.

Original languageEnglish (US)
Pages (from-to)739-749
Number of pages11
JournalImmunity
Volume6
Issue number6
DOIs
StatePublished - Jan 1 1997

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MAP Kinase Kinase 6
Peptide Hydrolases
Apoptosis
T-Lymphocytes
Cell Death
Caspase 1
Jurkat Cells
Mitogen-Activated Protein Kinase Kinases
Gene Expression Regulation
p38 Mitogen-Activated Protein Kinases
Ligation
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Apoptosis signaling pathway in T cells is composed of ICE/Ced-3 family proteases and MAP kinase kinase 6b. / Huang, Shuang; Jiang, Yong; Li, Zhuangjie; Nishida, Eisuke; Mathias, Patricia; Lin, Shengcai; Ulevitch, Richard J.; Nemerow, Glen R.; Han, Jiahuai.

In: Immunity, Vol. 6, No. 6, 01.01.1997, p. 739-749.

Research output: Contribution to journalArticle

Huang, S, Jiang, Y, Li, Z, Nishida, E, Mathias, P, Lin, S, Ulevitch, RJ, Nemerow, GR & Han, J 1997, 'Apoptosis signaling pathway in T cells is composed of ICE/Ced-3 family proteases and MAP kinase kinase 6b', Immunity, vol. 6, no. 6, pp. 739-749. https://doi.org/10.1016/S1074-7613(00)80449-5
Huang, Shuang ; Jiang, Yong ; Li, Zhuangjie ; Nishida, Eisuke ; Mathias, Patricia ; Lin, Shengcai ; Ulevitch, Richard J. ; Nemerow, Glen R. ; Han, Jiahuai. / Apoptosis signaling pathway in T cells is composed of ICE/Ced-3 family proteases and MAP kinase kinase 6b. In: Immunity. 1997 ; Vol. 6, No. 6. pp. 739-749.
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