Arginase 1 contributes to diminished coronary arteriolar dilation in patients with diabetes

Timea Beleznai, Attila Feher, David Spielvogel, Steven L. Lansman, Zsolt Bagi

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Arginase 1, via competing with nitric oxide (NO) synthase for the substrate L-arginine, may interfere with NO-mediated vascular responses. We tested the hypothesis that arginase 1 contributes to coronary vasomotor dysfunction in patients with diabetes mellitus (DM). Coronary arterioles were dissected from the right atrial appendages of 41 consecutive patients with or without DM (the 2 groups suffered from similar comorbidities), and agonist-induced changes in diameter were measured with videomicroscopy. We found that the endothelium-dependent agonist ACh elicited a diminished vasodilation and caused constriction to the highest ACh concentration (0.1 μM) with a similar magnitude in patients with (18 ± 8%) and without (17 ± 9%) DM. Responses to ACh were not significantly affected by the inhibition of NO synthesis with NG-nitro-L-arginine methyl ester in either group. The NO donor sodium nitroprusside-dependent dilations were not different in patients with or without DM. Interestingly, we found that the presence of NG-hydroxy-L-arginine (10 μM), a selective inhibitor of arginase or application of L-arginine (3 mM), restored ACh-induced coronary dilations only in patients with DM (to 47 ± 6% and to 40 ± 19%, respectively) but not in subjects without DM. Correspondingly, the protein expression of arginase 1 was increased in coronary arterioles of patients with DM compared with subjects without diabetes. Moreover, using immunocytochemistry, we detected an abundant immunostaining of arginase 1 in coronary endothelial cells of patients with DM, which was colocalized with NO synthase. Collectively, we provided evidence for a distinct upregulation of arginase 1 in coronary arterioles of patients with DM, which contributes to a reduced NO production and consequently diminished vasodilation.

Original languageEnglish (US)
Pages (from-to)H777-H783
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume300
Issue number3
DOIs
StatePublished - Mar 1 2011

Fingerprint

Arginase
Dilatation
Diabetes Mellitus
Arterioles
Arginine
Nitric Oxide
Vasodilation
Nitric Oxide Synthase
Atrial Appendage
Video Microscopy
Nitric Oxide Donors
NG-Nitroarginine Methyl Ester
Nitroprusside
Constriction
Endothelium
Blood Vessels
Comorbidity
Up-Regulation
Endothelial Cells
Immunohistochemistry

Keywords

  • Coronary microvessel
  • Endothelial nitric oxide synthase
  • Endothelium

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Arginase 1 contributes to diminished coronary arteriolar dilation in patients with diabetes. / Beleznai, Timea; Feher, Attila; Spielvogel, David; Lansman, Steven L.; Bagi, Zsolt.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 300, No. 3, 01.03.2011, p. H777-H783.

Research output: Contribution to journalArticle

Beleznai, Timea ; Feher, Attila ; Spielvogel, David ; Lansman, Steven L. ; Bagi, Zsolt. / Arginase 1 contributes to diminished coronary arteriolar dilation in patients with diabetes. In: American Journal of Physiology - Heart and Circulatory Physiology. 2011 ; Vol. 300, No. 3. pp. H777-H783.
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