Arginase 2 deficiency reduces hyperoxia-mediated retinal neurodegeneration through the regulation of polyamine metabolism

Subhadra Priyadarshini Narayanan, Z. Xu, N. Putluri, A. Sreekumar, T. Lemtalsi, Robert William Caldwell, Ruth B Caldwell

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Hyperoxia treatment has been known to induce neuronal and glial death in the developing central nervous system. Retinopathy of prematurity (ROP) is a devastating disease in premature infants and a major cause of childhood vision impairment. Studies indicate that, in addition to vascular injury, retinal neurons are also affected in ROP. Using an oxygen-induced retinopathy (OIR) mouse model for ROP, we have previously shown that deletion of the arginase 2 (A2) significantly reduced neuro-glial injury and improved retinal function. In the current study, we investigated the mechanism of A2 deficiency-mediated neuroprotection in the OIR retina. Hyperoxia treatment has been known to induce neuronal death in neonates. During the hyperoxia phase of OIR, a significant increase in the number of apoptotic cells was observed in the wild-type (WT) OIR retina compared with A2-deficient OIR. Mass spectrometric analysis showed alterations in polyamine metabolism in WT OIR retina. Further, increased expression level of spermine oxidase was observed in WT OIR retina, suggesting increased oxidation of polyamines in OIR retina. These changes were minimal in A2-deficient OIR retina. Treatment using the polyamine oxidase inhibitor, N, N′-bis (2, 3-butadienyl)-1, 4-butanediamine dihydrochloride, significantly improved neuronal survival during OIR treatment. Our data suggest that retinal arginase is involved in the hyperoxia-induced neuronal degeneration in the OIR model, through the regulation of polyamine metabolism.

Original languageEnglish (US)
Article numbere1075
JournalCell Death and Disease
Volume5
Issue number2
DOIs
StatePublished - Feb 1 2014

Fingerprint

Hyperargininemia
Hyperoxia
Polyamines
Oxygen
Retina
Arginase
Retinopathy of Prematurity
Neuroglia
Infant, Premature, Diseases
Retinal Neurons
Vascular System Injuries
Therapeutics

Keywords

  • arginase
  • hyperoxia
  • neurodegeneration
  • polyamine
  • retinopathy of prematurity

ASJC Scopus subject areas

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

Cite this

Arginase 2 deficiency reduces hyperoxia-mediated retinal neurodegeneration through the regulation of polyamine metabolism. / Narayanan, Subhadra Priyadarshini; Xu, Z.; Putluri, N.; Sreekumar, A.; Lemtalsi, T.; Caldwell, Robert William; Caldwell, Ruth B.

In: Cell Death and Disease, Vol. 5, No. 2, e1075, 01.02.2014.

Research output: Contribution to journalArticle

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