Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling

Yingjie Wang, Mi Zhou, Xiaolong Wang, Gangjian Qin, Neal L. Weintraub, Yaoliang Tang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Near-infrared fluorescence (NIRF) imaging by using infrared fluorescent protein (iRFP) gene labelling is a novel technology with potential value for in vivo applications. In this study, we expressed iRFP in mouse cardiac progenitor cells (CPC) by lentiviral vector and demonstrated that the iRFP-labelled CPC (CPCiRFP) can be detected by flow cytometry and fluorescent microscopy. We observed a linear correlation in vitro between cell numbers and infrared signal intensity by using the multiSpectral imaging system. CPCiRFP injected into the non-ischaemic mouse hindlimb were also readily detected by whole-animal NIRF imaging. We then compared iRFP against green fluorescent protein (GFP) for tracking survival of engrafted CPC in mouse ischaemic heart tissue. GFP-labelled CPC (CPCGFP) or CPC labelled with both iRFP and GFP (CPCiRFP GFP) were injected intramyocardially into mouse hearts after infarction. Three days after cell transplantation, a strong NIRF signal was detected in hearts into which CPCiRFP GFP, but not CPCGFP, were transplanted. Furthermore, iRFP fluorescence from engrafted CPCiRFP GFP was detected in tissue sections by confocal microscopy. In conclusion, the iRFP-labelling system provides a valuable molecular imaging tool to track the fate of transplanted progenitor cells in vivo.

Original languageEnglish (US)
Pages (from-to)1889-1894
Number of pages6
JournalJournal of Cellular and Molecular Medicine
Volume18
Issue number9
DOIs
StatePublished - Sep 1 2014

Fingerprint

Cell Tracking
Green Fluorescent Proteins
Stem Cells
Proteins
Optical Imaging
Fluorescence
Molecular Imaging
Cell Transplantation
Hindlimb
Confocal Microscopy
Infarction
In Vitro Techniques
Microscopy
Flow Cytometry
Cell Count
Technology

Keywords

  • Cell transplantation
  • GFP
  • IRFP
  • Myocardial infarction
  • Stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

Cite this

Assessing in vitro stem-cell function and tracking engraftment of stem cells in ischaemic hearts by using novel iRFP gene labelling. / Wang, Yingjie; Zhou, Mi; Wang, Xiaolong; Qin, Gangjian; Weintraub, Neal L.; Tang, Yaoliang.

In: Journal of Cellular and Molecular Medicine, Vol. 18, No. 9, 01.09.2014, p. 1889-1894.

Research output: Contribution to journalArticle

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