Assessment of endothelial function is reproducible in patients with cystic fibrosis

Cassandra C. Derella, Nichole Lee, Reva Crandall, Marsha Blackburn, Jacob Looney, Amie Mangieri, Paula Rodriguez-Miguelez, Matthew A. Tucker, Ryan A. Harris

Research output: Contribution to journalArticle

Abstract

Background: New treatments have improved life-expectancy in patients with cystic fibrosis (CF); however, cardiovascular health remains an area of concern in this population. Flow-mediated dilation (FMD), a non-invasive assessment of vascular endothelial function that predicts future cardiovascular disease and events, is attenuated in patients with CF compared to controls. The reproducibility of FMD in CF; however, has yet to be evaluated. Thus, this study sought to examine the within-day, between-day, and between-month reproducibility of FMD in patients with CF. Methods: Pulmonary function, baseline diameter (cm), peak diameter (cm), and FMD(%) were assessed 5 times (sessions A-E) over four visits in 13 patients with CF (six males, seven females, age range: 13–43 years old; mean forced expiratory volume in 1 s = 71% predicted). Sessions A and B (within-day), C (between-day), and D and E (between-month) were separated by 3 h, at least 10 days, and ~3 months, respectively. Reproducibility was assessed by: (1) paired t-tests, (2) coefficients of variation (CV), (3) CV prime, (4) Pearson's correlation (r), (5) intra-class correlation coefficient, and (6) Bland-Altman plots. Five acceptable parameters were required to determine reproducibility. Results: Pulmonary function was stable throughout all visits. FMD(%) and baseline diameter (cm) satisfied all six reproducibility criteria for within-day, while peak diameter (cm) met five of six criteria. All six reproducibility criteria were met for all between-day and between-month assessments. Conclusion: The present study provides evidence that endothelial function assessed by FMD is reproducible in patients with CF not only within-day, but also between-day and between-month.

Original languageEnglish (US)
JournalJournal of Cystic Fibrosis
DOIs
StatePublished - Jan 1 2019

Fingerprint

Cystic Fibrosis
Dilatation
Lung
Forced Expiratory Volume
Life Expectancy
Blood Vessels
Cardiovascular Diseases
Health
Population

Keywords

  • CVD risk
  • Flow-mediated dilation
  • Reproducibility
  • Vascular function

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine

Cite this

Assessment of endothelial function is reproducible in patients with cystic fibrosis. / Derella, Cassandra C.; Lee, Nichole; Crandall, Reva; Blackburn, Marsha; Looney, Jacob; Mangieri, Amie; Rodriguez-Miguelez, Paula; Tucker, Matthew A.; Harris, Ryan A.

In: Journal of Cystic Fibrosis, 01.01.2019.

Research output: Contribution to journalArticle

Derella, CC, Lee, N, Crandall, R, Blackburn, M, Looney, J, Mangieri, A, Rodriguez-Miguelez, P, Tucker, MA & Harris, RA 2019, 'Assessment of endothelial function is reproducible in patients with cystic fibrosis', Journal of Cystic Fibrosis. https://doi.org/10.1016/j.jcf.2019.03.011
Derella, Cassandra C. ; Lee, Nichole ; Crandall, Reva ; Blackburn, Marsha ; Looney, Jacob ; Mangieri, Amie ; Rodriguez-Miguelez, Paula ; Tucker, Matthew A. ; Harris, Ryan A. / Assessment of endothelial function is reproducible in patients with cystic fibrosis. In: Journal of Cystic Fibrosis. 2019.
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abstract = "Background: New treatments have improved life-expectancy in patients with cystic fibrosis (CF); however, cardiovascular health remains an area of concern in this population. Flow-mediated dilation (FMD), a non-invasive assessment of vascular endothelial function that predicts future cardiovascular disease and events, is attenuated in patients with CF compared to controls. The reproducibility of FMD in CF; however, has yet to be evaluated. Thus, this study sought to examine the within-day, between-day, and between-month reproducibility of FMD in patients with CF. Methods: Pulmonary function, baseline diameter (cm), peak diameter (cm), and FMD({\%}) were assessed 5 times (sessions A-E) over four visits in 13 patients with CF (six males, seven females, age range: 13–43 years old; mean forced expiratory volume in 1 s = 71{\%} predicted). Sessions A and B (within-day), C (between-day), and D and E (between-month) were separated by 3 h, at least 10 days, and ~3 months, respectively. Reproducibility was assessed by: (1) paired t-tests, (2) coefficients of variation (CV), (3) CV prime, (4) Pearson's correlation (r), (5) intra-class correlation coefficient, and (6) Bland-Altman plots. Five acceptable parameters were required to determine reproducibility. Results: Pulmonary function was stable throughout all visits. FMD({\%}) and baseline diameter (cm) satisfied all six reproducibility criteria for within-day, while peak diameter (cm) met five of six criteria. All six reproducibility criteria were met for all between-day and between-month assessments. Conclusion: The present study provides evidence that endothelial function assessed by FMD is reproducible in patients with CF not only within-day, but also between-day and between-month.",
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AU - Derella, Cassandra C.

AU - Lee, Nichole

AU - Crandall, Reva

AU - Blackburn, Marsha

AU - Looney, Jacob

AU - Mangieri, Amie

AU - Rodriguez-Miguelez, Paula

AU - Tucker, Matthew A.

AU - Harris, Ryan A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background: New treatments have improved life-expectancy in patients with cystic fibrosis (CF); however, cardiovascular health remains an area of concern in this population. Flow-mediated dilation (FMD), a non-invasive assessment of vascular endothelial function that predicts future cardiovascular disease and events, is attenuated in patients with CF compared to controls. The reproducibility of FMD in CF; however, has yet to be evaluated. Thus, this study sought to examine the within-day, between-day, and between-month reproducibility of FMD in patients with CF. Methods: Pulmonary function, baseline diameter (cm), peak diameter (cm), and FMD(%) were assessed 5 times (sessions A-E) over four visits in 13 patients with CF (six males, seven females, age range: 13–43 years old; mean forced expiratory volume in 1 s = 71% predicted). Sessions A and B (within-day), C (between-day), and D and E (between-month) were separated by 3 h, at least 10 days, and ~3 months, respectively. Reproducibility was assessed by: (1) paired t-tests, (2) coefficients of variation (CV), (3) CV prime, (4) Pearson's correlation (r), (5) intra-class correlation coefficient, and (6) Bland-Altman plots. Five acceptable parameters were required to determine reproducibility. Results: Pulmonary function was stable throughout all visits. FMD(%) and baseline diameter (cm) satisfied all six reproducibility criteria for within-day, while peak diameter (cm) met five of six criteria. All six reproducibility criteria were met for all between-day and between-month assessments. Conclusion: The present study provides evidence that endothelial function assessed by FMD is reproducible in patients with CF not only within-day, but also between-day and between-month.

AB - Background: New treatments have improved life-expectancy in patients with cystic fibrosis (CF); however, cardiovascular health remains an area of concern in this population. Flow-mediated dilation (FMD), a non-invasive assessment of vascular endothelial function that predicts future cardiovascular disease and events, is attenuated in patients with CF compared to controls. The reproducibility of FMD in CF; however, has yet to be evaluated. Thus, this study sought to examine the within-day, between-day, and between-month reproducibility of FMD in patients with CF. Methods: Pulmonary function, baseline diameter (cm), peak diameter (cm), and FMD(%) were assessed 5 times (sessions A-E) over four visits in 13 patients with CF (six males, seven females, age range: 13–43 years old; mean forced expiratory volume in 1 s = 71% predicted). Sessions A and B (within-day), C (between-day), and D and E (between-month) were separated by 3 h, at least 10 days, and ~3 months, respectively. Reproducibility was assessed by: (1) paired t-tests, (2) coefficients of variation (CV), (3) CV prime, (4) Pearson's correlation (r), (5) intra-class correlation coefficient, and (6) Bland-Altman plots. Five acceptable parameters were required to determine reproducibility. Results: Pulmonary function was stable throughout all visits. FMD(%) and baseline diameter (cm) satisfied all six reproducibility criteria for within-day, while peak diameter (cm) met five of six criteria. All six reproducibility criteria were met for all between-day and between-month assessments. Conclusion: The present study provides evidence that endothelial function assessed by FMD is reproducible in patients with CF not only within-day, but also between-day and between-month.

KW - CVD risk

KW - Flow-mediated dilation

KW - Reproducibility

KW - Vascular function

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