Assessment of endothelial function is reproducible in patients with cystic fibrosis

Cassandra C. Derella, Nichole Lee, Reva Crandall, Marsha Blackburn, Jacob Looney, Amie Mangieri, Paula Rodriguez-Miguelez, Matthew A. Tucker, Ryan A. Harris

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background: New treatments have improved life-expectancy in patients with cystic fibrosis (CF); however, cardiovascular health remains an area of concern in this population. Flow-mediated dilation (FMD), a non-invasive assessment of vascular endothelial function that predicts future cardiovascular disease and events, is attenuated in patients with CF compared to controls. The reproducibility of FMD in CF; however, has yet to be evaluated. Thus, this study sought to examine the within-day, between-day, and between-month reproducibility of FMD in patients with CF. Methods: Pulmonary function, baseline diameter (cm), peak diameter (cm), and FMD(%) were assessed 5 times (sessions A-E) over four visits in 13 patients with CF (six males, seven females, age range: 13–43 years old; mean forced expiratory volume in 1 s = 71% predicted). Sessions A and B (within-day), C (between-day), and D and E (between-month) were separated by 3 h, at least 10 days, and ~3 months, respectively. Reproducibility was assessed by: (1) paired t-tests, (2) coefficients of variation (CV), (3) CV prime, (4) Pearson's correlation (r), (5) intra-class correlation coefficient, and (6) Bland-Altman plots. Five acceptable parameters were required to determine reproducibility. Results: Pulmonary function was stable throughout all visits. FMD(%) and baseline diameter (cm) satisfied all six reproducibility criteria for within-day, while peak diameter (cm) met five of six criteria. All six reproducibility criteria were met for all between-day and between-month assessments. Conclusion: The present study provides evidence that endothelial function assessed by FMD is reproducible in patients with CF not only within-day, but also between-day and between-month.

Original languageEnglish (US)
Pages (from-to)772-777
Number of pages6
JournalJournal of Cystic Fibrosis
Volume18
Issue number6
DOIs
StatePublished - Nov 2019

Keywords

  • CVD risk
  • Flow-mediated dilation
  • Reproducibility
  • Vascular function

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pulmonary and Respiratory Medicine

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  • Cite this

    Derella, C. C., Lee, N., Crandall, R., Blackburn, M., Looney, J., Mangieri, A., Rodriguez-Miguelez, P., Tucker, M. A., & Harris, R. A. (2019). Assessment of endothelial function is reproducible in patients with cystic fibrosis. Journal of Cystic Fibrosis, 18(6), 772-777. https://doi.org/10.1016/j.jcf.2019.03.011