Assessment of lipopolysaccharide and outer membrane of Bacteroides fragilis by an antibody-inhibition enzyme-linked immunosorbent assay in physiologic fluids and infected animals

J. Peter Rissing, Thomas B. Buxton, Richard Harris, William Moore

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Abstract

LPS antigen of Bacteroides fragilis (CDC strain 5462) was measured in vitro in physiologic buffer and undilute human sera by using an antibody-inhibition ELISA system. Other studies were performed to assess detection of the outer membrane antigen from this organism. LPS was repetitively detected at 20 to 50 ng/ml dry weight, and outer membranes were detected at 200 ng/ml total protein in physiologic buffers and human sera. LPS of other type strains was also detected. Prior incubation of the reagent antibody with multiple whole Enterobacteriaceae organisms and Pseudomonas aeruginosa did not alter test results. Bacteremic rats were easily separated into those with B. fragilis (N = 15) and Escherichia coli (N = 14) bacteremias. Sera from rats in which subcutaneous abscesses were produced with 18 strains of Enterobacteriaceae inhibited detection antibody significantly less than did sera from 30 rats in which abscesses were produced with 11 strains of B. fragilis (p < 0.01). Although values from the group of animals challenged with B. fragilis were significantly different from the group challenged with Enterobacteriaceae, the present results lack significant sensitivity and specificity for clinical application.

Original languageEnglish (US)
Pages (from-to)784-794
Number of pages11
JournalThe Journal of Laboratory and Clinical Medicine
Volume98
Issue number5
StatePublished - Nov 1981

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Enzyme inhibition
Bacteroides fragilis
Immunosorbents
Lipopolysaccharides
Rats
Assays
Animals
Enterobacteriaceae
Enzyme-Linked Immunosorbent Assay
Membranes
Fluids
Antibodies
Buffers
Serum
Abscess
Antigens
Escherichia coli
Centers for Disease Control and Prevention (U.S.)
Bacteremia
Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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title = "Assessment of lipopolysaccharide and outer membrane of Bacteroides fragilis by an antibody-inhibition enzyme-linked immunosorbent assay in physiologic fluids and infected animals",
abstract = "LPS antigen of Bacteroides fragilis (CDC strain 5462) was measured in vitro in physiologic buffer and undilute human sera by using an antibody-inhibition ELISA system. Other studies were performed to assess detection of the outer membrane antigen from this organism. LPS was repetitively detected at 20 to 50 ng/ml dry weight, and outer membranes were detected at 200 ng/ml total protein in physiologic buffers and human sera. LPS of other type strains was also detected. Prior incubation of the reagent antibody with multiple whole Enterobacteriaceae organisms and Pseudomonas aeruginosa did not alter test results. Bacteremic rats were easily separated into those with B. fragilis (N = 15) and Escherichia coli (N = 14) bacteremias. Sera from rats in which subcutaneous abscesses were produced with 18 strains of Enterobacteriaceae inhibited detection antibody significantly less than did sera from 30 rats in which abscesses were produced with 11 strains of B. fragilis (p < 0.01). Although values from the group of animals challenged with B. fragilis were significantly different from the group challenged with Enterobacteriaceae, the present results lack significant sensitivity and specificity for clinical application.",
author = "Rissing, {J. Peter} and Buxton, {Thomas B.} and Richard Harris and William Moore",
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T1 - Assessment of lipopolysaccharide and outer membrane of Bacteroides fragilis by an antibody-inhibition enzyme-linked immunosorbent assay in physiologic fluids and infected animals

AU - Rissing, J. Peter

AU - Buxton, Thomas B.

AU - Harris, Richard

AU - Moore, William

PY - 1981/11

Y1 - 1981/11

N2 - LPS antigen of Bacteroides fragilis (CDC strain 5462) was measured in vitro in physiologic buffer and undilute human sera by using an antibody-inhibition ELISA system. Other studies were performed to assess detection of the outer membrane antigen from this organism. LPS was repetitively detected at 20 to 50 ng/ml dry weight, and outer membranes were detected at 200 ng/ml total protein in physiologic buffers and human sera. LPS of other type strains was also detected. Prior incubation of the reagent antibody with multiple whole Enterobacteriaceae organisms and Pseudomonas aeruginosa did not alter test results. Bacteremic rats were easily separated into those with B. fragilis (N = 15) and Escherichia coli (N = 14) bacteremias. Sera from rats in which subcutaneous abscesses were produced with 18 strains of Enterobacteriaceae inhibited detection antibody significantly less than did sera from 30 rats in which abscesses were produced with 11 strains of B. fragilis (p < 0.01). Although values from the group of animals challenged with B. fragilis were significantly different from the group challenged with Enterobacteriaceae, the present results lack significant sensitivity and specificity for clinical application.

AB - LPS antigen of Bacteroides fragilis (CDC strain 5462) was measured in vitro in physiologic buffer and undilute human sera by using an antibody-inhibition ELISA system. Other studies were performed to assess detection of the outer membrane antigen from this organism. LPS was repetitively detected at 20 to 50 ng/ml dry weight, and outer membranes were detected at 200 ng/ml total protein in physiologic buffers and human sera. LPS of other type strains was also detected. Prior incubation of the reagent antibody with multiple whole Enterobacteriaceae organisms and Pseudomonas aeruginosa did not alter test results. Bacteremic rats were easily separated into those with B. fragilis (N = 15) and Escherichia coli (N = 14) bacteremias. Sera from rats in which subcutaneous abscesses were produced with 18 strains of Enterobacteriaceae inhibited detection antibody significantly less than did sera from 30 rats in which abscesses were produced with 11 strains of B. fragilis (p < 0.01). Although values from the group of animals challenged with B. fragilis were significantly different from the group challenged with Enterobacteriaceae, the present results lack significant sensitivity and specificity for clinical application.

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