Association between the C825T polymorphism of the G protein β3-subunit gene and hypertension in blacks

Yanbin Dong, Haidong Zhu, Giuseppe A. Sagnella, Nicholas D. Carter, Derek G. Cook, Francesco P. Cappuccio

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

A polymorphism (C825T) of the G protein β3-subunit gene has been associated with low renin hypertension in whites. The aim of this study was to examine the C825T polymorphism in relation to hypertension in a population-based study of black people of African origin who have high prevalence of low renin, salt-sensitive hypertension. A total of 428 men and women, aged 40 to 59 years (270 Caribbeans and 158 West Africans), who took part in a population-based survey were studied. All were blacks and first- generation immigrants. The C825T polymorphism was detected by polymerase chain reaction followed by restriction-enzyme digestion. The prevalence of hypertension (supine blood pressures ≥ 160 systolic and/or 95 mm Hg diastolic or on drug therapy) was 43%. The distribution of the genotypes (CC, CT, and TT) was in Hardy-Weinberg equilibrium with observed frequencies of 4.0% (n=17), 33.6% (n=144), and 62.4% (n=267), respectively. Allele frequencies were 20.8% for C and 79.2% for T. No difference was detected between Caribbeans and West Africans. A 3-fold higher risk of hypertension was found among the carriers of the T variant both as heterozygotes (odds ratio [OR], 3.43 [95% CI, 0.94 to 12.4]) and homozygotes (OR, 3.87 [95% CI, 1.09 to 13.8]). The estimate of effect and the blood pressure values in the groups carrying the T variant suggested a dominant model for the T allele. This was confirmed by a significant association between the T allele and hypertension (OR, 3.71 [95% CI, 1.05 to 13.1]), even when adjusted for age, sex, and body mass index (OR, 4.14 [95% CI, 1.11 to 15.4]). The study shows, for the first time, a high frequency of the 825T allele in black people, and it provides evidence that the T allele may be a susceptibility factor for the development of hypertension in blacks. Given the high frequency of the T allele, even a 2-fold increased risk of hypertension among the carriers of the T allele might account for 44% of the cases of hypertension in blacks.

Original languageEnglish (US)
Pages (from-to)1193-1196
Number of pages4
JournalHypertension
Volume34
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

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Protein Subunits
GTP-Binding Proteins
Hypertension
Genes
Alleles
Odds Ratio
Gene Frequency
Renin
Blood Pressure
Homozygote
Heterozygote
Population
Digestion
Body Mass Index
Salts
Genotype
Drug Therapy
Polymerase Chain Reaction
Enzymes

Keywords

  • Blacks
  • Epidemiology
  • G protein
  • Hypertension, genetic
  • Polymorphism

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Association between the C825T polymorphism of the G protein β3-subunit gene and hypertension in blacks. / Dong, Yanbin; Zhu, Haidong; Sagnella, Giuseppe A.; Carter, Nicholas D.; Cook, Derek G.; Cappuccio, Francesco P.

In: Hypertension, Vol. 34, No. 6, 01.01.1999, p. 1193-1196.

Research output: Contribution to journalArticle

Dong, Yanbin ; Zhu, Haidong ; Sagnella, Giuseppe A. ; Carter, Nicholas D. ; Cook, Derek G. ; Cappuccio, Francesco P. / Association between the C825T polymorphism of the G protein β3-subunit gene and hypertension in blacks. In: Hypertension. 1999 ; Vol. 34, No. 6. pp. 1193-1196.
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abstract = "A polymorphism (C825T) of the G protein β3-subunit gene has been associated with low renin hypertension in whites. The aim of this study was to examine the C825T polymorphism in relation to hypertension in a population-based study of black people of African origin who have high prevalence of low renin, salt-sensitive hypertension. A total of 428 men and women, aged 40 to 59 years (270 Caribbeans and 158 West Africans), who took part in a population-based survey were studied. All were blacks and first- generation immigrants. The C825T polymorphism was detected by polymerase chain reaction followed by restriction-enzyme digestion. The prevalence of hypertension (supine blood pressures ≥ 160 systolic and/or 95 mm Hg diastolic or on drug therapy) was 43{\%}. The distribution of the genotypes (CC, CT, and TT) was in Hardy-Weinberg equilibrium with observed frequencies of 4.0{\%} (n=17), 33.6{\%} (n=144), and 62.4{\%} (n=267), respectively. Allele frequencies were 20.8{\%} for C and 79.2{\%} for T. No difference was detected between Caribbeans and West Africans. A 3-fold higher risk of hypertension was found among the carriers of the T variant both as heterozygotes (odds ratio [OR], 3.43 [95{\%} CI, 0.94 to 12.4]) and homozygotes (OR, 3.87 [95{\%} CI, 1.09 to 13.8]). The estimate of effect and the blood pressure values in the groups carrying the T variant suggested a dominant model for the T allele. This was confirmed by a significant association between the T allele and hypertension (OR, 3.71 [95{\%} CI, 1.05 to 13.1]), even when adjusted for age, sex, and body mass index (OR, 4.14 [95{\%} CI, 1.11 to 15.4]). The study shows, for the first time, a high frequency of the 825T allele in black people, and it provides evidence that the T allele may be a susceptibility factor for the development of hypertension in blacks. Given the high frequency of the T allele, even a 2-fold increased risk of hypertension among the carriers of the T allele might account for 44{\%} of the cases of hypertension in blacks.",
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AU - Cook, Derek G.

AU - Cappuccio, Francesco P.

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N2 - A polymorphism (C825T) of the G protein β3-subunit gene has been associated with low renin hypertension in whites. The aim of this study was to examine the C825T polymorphism in relation to hypertension in a population-based study of black people of African origin who have high prevalence of low renin, salt-sensitive hypertension. A total of 428 men and women, aged 40 to 59 years (270 Caribbeans and 158 West Africans), who took part in a population-based survey were studied. All were blacks and first- generation immigrants. The C825T polymorphism was detected by polymerase chain reaction followed by restriction-enzyme digestion. The prevalence of hypertension (supine blood pressures ≥ 160 systolic and/or 95 mm Hg diastolic or on drug therapy) was 43%. The distribution of the genotypes (CC, CT, and TT) was in Hardy-Weinberg equilibrium with observed frequencies of 4.0% (n=17), 33.6% (n=144), and 62.4% (n=267), respectively. Allele frequencies were 20.8% for C and 79.2% for T. No difference was detected between Caribbeans and West Africans. A 3-fold higher risk of hypertension was found among the carriers of the T variant both as heterozygotes (odds ratio [OR], 3.43 [95% CI, 0.94 to 12.4]) and homozygotes (OR, 3.87 [95% CI, 1.09 to 13.8]). The estimate of effect and the blood pressure values in the groups carrying the T variant suggested a dominant model for the T allele. This was confirmed by a significant association between the T allele and hypertension (OR, 3.71 [95% CI, 1.05 to 13.1]), even when adjusted for age, sex, and body mass index (OR, 4.14 [95% CI, 1.11 to 15.4]). The study shows, for the first time, a high frequency of the 825T allele in black people, and it provides evidence that the T allele may be a susceptibility factor for the development of hypertension in blacks. Given the high frequency of the T allele, even a 2-fold increased risk of hypertension among the carriers of the T allele might account for 44% of the cases of hypertension in blacks.

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