Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Qingqing Wei; Xiaoyang Cheng; Keying Chen; Jun Hu; Minqian Li; Peihong Zhu

doi:10.1360/02yc9025

Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Qingqing Wei, Xiaoyang Cheng, Keying Chen, Jun Hu, Minqian Li, Peihong Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca²⁺ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

Original language	English (US)
Pages (from-to)	225-236
Number of pages	12
Journal	Science in China, Series C: Life Sciences
Volume	45
Issue number	3
DOIs	https://doi.org/10.1360/02yc9025
State	Published - 2002
Externally published	Yes

Keywords

Atomic force microscope
Elasticity
Ryanodine receptor/calcium release channel

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Environmental Science(all)
Agricultural and Biological Sciences(all)

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10.1360/02yc9025

Cite this

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title = "Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states",

abstract = "Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.",

keywords = "Atomic force microscope, Elasticity, Ryanodine receptor/calcium release channel",

author = "Qingqing Wei and Xiaoyang Cheng and Keying Chen and Jun Hu and Minqian Li and Peihong Zhu",

note = "Funding Information: Acknowledgements This work was supported by the Ninth Five-Year Major Research Programs of Chinese Academy of Sciences and the Major National Basic Research Program.",

year = "2002",

doi = "10.1360/02yc9025",

language = "English (US)",

volume = "45",

pages = "225--236",

journal = "Science in China, Series C: Life Sciences",

issn = "1006-9305",

publisher = "Science in China Press",

number = "3",

}

TY - JOUR

T1 - Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

AU - Wei, Qingqing

AU - Cheng, Xiaoyang

AU - Chen, Keying

AU - Hu, Jun

AU - Li, Minqian

AU - Zhu, Peihong

N1 - Funding Information: Acknowledgements This work was supported by the Ninth Five-Year Major Research Programs of Chinese Academy of Sciences and the Major National Basic Research Program.

PY - 2002

Y1 - 2002

N2 - Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

AB - Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

KW - Atomic force microscope

KW - Elasticity

KW - Ryanodine receptor/calcium release channel

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DO - 10.1360/02yc9025

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AN - SCOPUS:33646768160

SN - 1006-9305

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SP - 225

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JO - Science in China, Series C: Life Sciences

JF - Science in China, Series C: Life Sciences

IS - 3

ER -

Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Abstract

Keywords

ASJC Scopus subject areas

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