Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

QingQing Wei, Xiaoyang Cheng, Keying Chen, Jun Hu, Minqian Li, Peihong Zhu

Research output: Contribution to journalArticle

Abstract

Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalScience in China, Series C: Life Sciences
Volume45
Issue number3
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

ryanodine receptors
Ryanodine Receptor Calcium Release Channel
atomic force microscopy
Atomic Force Microscopy
phospholipid
Muscle
skeletal muscle
Atomic force microscopy
Phospholipids
phospholipids
Skeletal Muscle
muscle
Elastic Modulus
rabbits
Young modulus
Rabbits
modulus of elasticity
Elastic moduli
calcium
Elasticity

Keywords

  • Atomic force microscope
  • Elasticity
  • Ryanodine receptor/calcium release channel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

Cite this

Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states. / Wei, QingQing; Cheng, Xiaoyang; Chen, Keying; Hu, Jun; Li, Minqian; Zhu, Peihong.

In: Science in China, Series C: Life Sciences, Vol. 45, No. 3, 01.12.2002, p. 225-236.

Research output: Contribution to journalArticle

Wei, QingQing ; Cheng, Xiaoyang ; Chen, Keying ; Hu, Jun ; Li, Minqian ; Zhu, Peihong. / Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states. In: Science in China, Series C: Life Sciences. 2002 ; Vol. 45, No. 3. pp. 225-236.
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