Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states

Qingqing Wei, Xiaoyang Cheng, Keying Chen, Jun Hu, Minqian Li, Peihong Zhu

Research output: Contribution to journalArticle


Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors.

Original languageEnglish (US)
Pages (from-to)225-236
Number of pages12
JournalScience in China, Series C: Life Sciences
Issue number3
StatePublished - Dec 1 2002
Externally publishedYes



  • Atomic force microscope
  • Elasticity
  • Ryanodine receptor/calcium release channel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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