Abstract
Formation of an aberrant, chimeric Bcr-Abl protein is the hallmark of Philadelphia (Ph) chromosome-positive leukemias. The Bcr-Abl protein, as well as its normal cellular counterparts - Abl and Bcr - are intracellular molecules with postulated roles in a variety of critical biologic functions. In this study, we demonstrate the existence of autoantibodies against these proteins. Plasma from 18 of 31 individuals (58%), including 14 of 20 Ph-positive CML patients (70%), two of four normal volunteers (50%), and two of seven patients with Ph-negative leukemia (29%) recognized p210(Bcr-Abl) when used in immunoprecipitation followed by immunoblotting experiments. In all 18 patients, plasma was able to recognize baculovirus-expressed Abl protein; in four patients, recognition of baculovirus-expressed Bcr protein was also demonstrated. These observations suggest that a humoral immune response to p210(Bcr-Abl) is discernible in both Ph-positive and -negative leukemias and in healthy individuals, and is most likely due to autoantibodies which recognize normal Abl and, to a lesser extent, normal Bcr proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1661-1666 |
| Number of pages | 6 |
| Journal | Leukemia |
| Volume | 14 |
| Issue number | 9 |
| State | Published - Jan 1 2000 |
| Externally published | Yes |
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Keywords
- Abl
- Antibody
- Bcr
- Chronic myelogenous leukemia
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research
Cite this
Autoantibodies to Abl and Bcr proteins. / Talpaz, M.; Qiu, X.; Cheng, K.; Cortes, J. E.; Kantarjian, H.; Kurzrock, R.
In: Leukemia, Vol. 14, No. 9, 01.01.2000, p. 1661-1666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Autoantibodies to Abl and Bcr proteins
AU - Talpaz, M.
AU - Qiu, X.
AU - Cheng, K.
AU - Cortes, J. E.
AU - Kantarjian, H.
AU - Kurzrock, R.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Formation of an aberrant, chimeric Bcr-Abl protein is the hallmark of Philadelphia (Ph) chromosome-positive leukemias. The Bcr-Abl protein, as well as its normal cellular counterparts - Abl and Bcr - are intracellular molecules with postulated roles in a variety of critical biologic functions. In this study, we demonstrate the existence of autoantibodies against these proteins. Plasma from 18 of 31 individuals (58%), including 14 of 20 Ph-positive CML patients (70%), two of four normal volunteers (50%), and two of seven patients with Ph-negative leukemia (29%) recognized p210(Bcr-Abl) when used in immunoprecipitation followed by immunoblotting experiments. In all 18 patients, plasma was able to recognize baculovirus-expressed Abl protein; in four patients, recognition of baculovirus-expressed Bcr protein was also demonstrated. These observations suggest that a humoral immune response to p210(Bcr-Abl) is discernible in both Ph-positive and -negative leukemias and in healthy individuals, and is most likely due to autoantibodies which recognize normal Abl and, to a lesser extent, normal Bcr proteins.
AB - Formation of an aberrant, chimeric Bcr-Abl protein is the hallmark of Philadelphia (Ph) chromosome-positive leukemias. The Bcr-Abl protein, as well as its normal cellular counterparts - Abl and Bcr - are intracellular molecules with postulated roles in a variety of critical biologic functions. In this study, we demonstrate the existence of autoantibodies against these proteins. Plasma from 18 of 31 individuals (58%), including 14 of 20 Ph-positive CML patients (70%), two of four normal volunteers (50%), and two of seven patients with Ph-negative leukemia (29%) recognized p210(Bcr-Abl) when used in immunoprecipitation followed by immunoblotting experiments. In all 18 patients, plasma was able to recognize baculovirus-expressed Abl protein; in four patients, recognition of baculovirus-expressed Bcr protein was also demonstrated. These observations suggest that a humoral immune response to p210(Bcr-Abl) is discernible in both Ph-positive and -negative leukemias and in healthy individuals, and is most likely due to autoantibodies which recognize normal Abl and, to a lesser extent, normal Bcr proteins.
KW - Abl
KW - Antibody
KW - Bcr
KW - Chronic myelogenous leukemia
UR - http://www.scopus.com/inward/record.url?scp=0033835428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033835428&partnerID=8YFLogxK
M3 - Article
C2 - 10995014
AN - SCOPUS:0033835428
VL - 14
SP - 1661
EP - 1666
JO - Leukemia
JF - Leukemia
SN - 0887-6924
IS - 9
ER -