Autoantibodies to Abl and Bcr proteins

M. Talpaz, X. Qiu, K. Cheng, J. E. Cortes, H. Kantarjian, R. Kurzrock

Research output: Contribution to journalArticle

Abstract

Formation of an aberrant, chimeric Bcr-Abl protein is the hallmark of Philadelphia (Ph) chromosome-positive leukemias. The Bcr-Abl protein, as well as its normal cellular counterparts - Abl and Bcr - are intracellular molecules with postulated roles in a variety of critical biologic functions. In this study, we demonstrate the existence of autoantibodies against these proteins. Plasma from 18 of 31 individuals (58%), including 14 of 20 Ph-positive CML patients (70%), two of four normal volunteers (50%), and two of seven patients with Ph-negative leukemia (29%) recognized p210(Bcr-Abl) when used in immunoprecipitation followed by immunoblotting experiments. In all 18 patients, plasma was able to recognize baculovirus-expressed Abl protein; in four patients, recognition of baculovirus-expressed Bcr protein was also demonstrated. These observations suggest that a humoral immune response to p210(Bcr-Abl) is discernible in both Ph-positive and -negative leukemias and in healthy individuals, and is most likely due to autoantibodies which recognize normal Abl and, to a lesser extent, normal Bcr proteins.

Original languageEnglish (US)
Pages (from-to)1661-1666
Number of pages6
JournalLeukemia
Volume14
Issue number9
StatePublished - Jan 1 2000
Externally publishedYes

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Autoantibodies
Leukemia
Proteins
Baculoviridae
Philadelphia Chromosome
Humoral Immunity
Immunoprecipitation
Immunoblotting
Healthy Volunteers

Keywords

  • Abl
  • Antibody
  • Bcr
  • Chronic myelogenous leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Talpaz, M., Qiu, X., Cheng, K., Cortes, J. E., Kantarjian, H., & Kurzrock, R. (2000). Autoantibodies to Abl and Bcr proteins. Leukemia, 14(9), 1661-1666.

Autoantibodies to Abl and Bcr proteins. / Talpaz, M.; Qiu, X.; Cheng, K.; Cortes, J. E.; Kantarjian, H.; Kurzrock, R.

In: Leukemia, Vol. 14, No. 9, 01.01.2000, p. 1661-1666.

Research output: Contribution to journalArticle

Talpaz, M, Qiu, X, Cheng, K, Cortes, JE, Kantarjian, H & Kurzrock, R 2000, 'Autoantibodies to Abl and Bcr proteins', Leukemia, vol. 14, no. 9, pp. 1661-1666.
Talpaz M, Qiu X, Cheng K, Cortes JE, Kantarjian H, Kurzrock R. Autoantibodies to Abl and Bcr proteins. Leukemia. 2000 Jan 1;14(9):1661-1666.
Talpaz, M. ; Qiu, X. ; Cheng, K. ; Cortes, J. E. ; Kantarjian, H. ; Kurzrock, R. / Autoantibodies to Abl and Bcr proteins. In: Leukemia. 2000 ; Vol. 14, No. 9. pp. 1661-1666.
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