BAL fluid contains detectable superoxide dismutase 1 activity

Robert A. DiSilvestro, Eric Pacht, W. Bruce Davis, Nizar Jarjour, Hyojee Joung, Kathleen Trela-Fulop

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: This study determined which, if any, of the three superoxide dismutase (SOD) enzyme activities were detectable in BAL fluid (BALF). Background: The identity and concentrations of antioxidant molecules in BALF have not been fully characterized. One important class of antioxidants is that of the SOD enzymes. Methods: BALF from control nonsmokers (n=9), smokers (n=7), and asthmatic subjects (n=12) were examined for SOD activity by a modified pyrogallol auto-oxidation method. The particular SOD enzyme or enzymes responsible for any activity were identified based on activity inhibition data and gel filtration column chromatography patterns. Results: SOD activity was detected in all samples. Unlike serum or some other fluids, in which the enzyme extracellular SOD accounts for virtually all SOD activity, the enzyme SOD 1 accounted for virtually all SOD activity. BALF SOD activities were lower for smokers than for control or asthmatic subjects (p<0.05). Conclusion: BALF SOD 1 activities can be measured as part of lung antioxidant studies. Data from a limited number of subjects suggested that smokers can have low BALF SOD values.

Original languageEnglish (US)
Pages (from-to)401-404
Number of pages4
JournalChest
Volume113
Issue number2
DOIs
StatePublished - Jan 1 1998

Fingerprint

Dimercaprol
Superoxide Dismutase
Enzymes
Antioxidants
Superoxide Dismutase-1
Pyrogallol
Gel Chromatography

Keywords

  • Antioxidants
  • Asthma
  • Bronchoalveolar lavage fluid
  • Smokers
  • Superoxide dismutase

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

DiSilvestro, R. A., Pacht, E., Davis, W. B., Jarjour, N., Joung, H., & Trela-Fulop, K. (1998). BAL fluid contains detectable superoxide dismutase 1 activity. Chest, 113(2), 401-404. https://doi.org/10.1378/chest.113.2.401

BAL fluid contains detectable superoxide dismutase 1 activity. / DiSilvestro, Robert A.; Pacht, Eric; Davis, W. Bruce; Jarjour, Nizar; Joung, Hyojee; Trela-Fulop, Kathleen.

In: Chest, Vol. 113, No. 2, 01.01.1998, p. 401-404.

Research output: Contribution to journalArticle

DiSilvestro, RA, Pacht, E, Davis, WB, Jarjour, N, Joung, H & Trela-Fulop, K 1998, 'BAL fluid contains detectable superoxide dismutase 1 activity', Chest, vol. 113, no. 2, pp. 401-404. https://doi.org/10.1378/chest.113.2.401
DiSilvestro RA, Pacht E, Davis WB, Jarjour N, Joung H, Trela-Fulop K. BAL fluid contains detectable superoxide dismutase 1 activity. Chest. 1998 Jan 1;113(2):401-404. https://doi.org/10.1378/chest.113.2.401
DiSilvestro, Robert A. ; Pacht, Eric ; Davis, W. Bruce ; Jarjour, Nizar ; Joung, Hyojee ; Trela-Fulop, Kathleen. / BAL fluid contains detectable superoxide dismutase 1 activity. In: Chest. 1998 ; Vol. 113, No. 2. pp. 401-404.
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