Basic concepts of molecular biology as applied to pediatric and adolescent gynecology

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Recombinant DNA techniques have increased our understanding of genetic disorders and facilitated their diagnosis and may eventually lead to therapy. Expression may be assessed and quantified by Northern blot analysis, whereas in situ hybridization may localize the transcript. Southern blotting has been the standard method to identify gene mutations and RFLPs, which are useful in the diagnosis of many different disorder. Special multiallelic RFLPs produce a DNA fingerprint particularly useful in paternity testing and forensic medicine. PCR has greatly reduced the time and expense to diagnose genetic disorders, and has an enormous number of applications both clinically and at the research level. These techniques along with DNA sequencing are currently being used in the human genome project, which may some day allow 'DNA typing' of individuals for any gene of interest. There is no doubt that new techniques in molecular biology will continue to evolve so that the goal of gene therapy for many disorders may be possible in the future.

Original languageEnglish (US)
Pages (from-to)1-26
Number of pages26
JournalObstetrics and Gynecology Clinics of North America
Volume19
Issue number1
StatePublished - Jan 1 1992

Fingerprint

Gynecology
Molecular Biology
Inborn Genetic Diseases
DNA Fingerprinting
Pediatrics
Restriction Fragment Length Polymorphisms
Human Genome Project
Forensic Medicine
Recombinant DNA
Southern Blotting
DNA Sequence Analysis
Northern Blotting
Genetic Therapy
Genes
In Situ Hybridization
Polymerase Chain Reaction
Mutation
Research
Therapeutics

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Basic concepts of molecular biology as applied to pediatric and adolescent gynecology. / Layman, Lawrence C.

In: Obstetrics and Gynecology Clinics of North America, Vol. 19, No. 1, 01.01.1992, p. 1-26.

Research output: Contribution to journalReview article

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