Beneficial effect of intracellular trehalose during mammalian cell storage

Tani Chen, Ali Eroglu, Alex Fowler, Stephen Cheley, Hagan Bayley, Mehmet Toner

Research output: Contribution to journalConference article

Abstract

Using a genetically engineered mutant of Staphylococcus aureus alpha-hemolysin to create pores in the cellular membrane, trehalose was successfully loaded into cells. Differential scanning calorimetry was used to study the glass transition temperature in cells containing intracellular trehalose. Results show higher glass transition temperatures and enhanced stability of the cytoplasm due to the presence of trehalose, compared to cells without intracellular trehalose. In addition, initial experiments show that cell survival depends on the glass transition temperature, as well as the storage temperature and the final moisture content.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - Dec 1 2000
Externally publishedYes
Event2000 Annual Fall Meeting of the Biomedical Engineering Society - Washington, WA, USA
Duration: Oct 12 2000Oct 14 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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  • Cite this

    Chen, T., Eroglu, A., Fowler, A., Cheley, S., Bayley, H., & Toner, M. (2000). Beneficial effect of intracellular trehalose during mammalian cell storage. Annals of Biomedical Engineering, 28(SUPPL. 1).