Berardinelli-Seip congenital lipodystrophy 2/seipin is not required for brown adipogenesis but regulates brown adipose tissue development and function

Hongyi Zhou, Stephen Matthew Black, Tyler W. Benson, Neal Lee Weintraub, Weiqin Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Brown adipose tissue (BAT) plays a unique role in regulating whole-body energy homeostasis by dissipating energy through thermogenic uncoupling. Berardinelli-Seip congenital lipodystrophy (BSCL) type 2 (BSCL2; also known as seipin) is a lipodystrophy- associated endoplasmic reticulum membrane protein essential for white adipocyte differentiation. Whether BSCL2 directly participates in brown adipocyte differentiation, development, and function, however, is unknown. We show that BSCL2 expression is increased during brown adipocyte differentiation. Its deletion does not impair the classic brown adipogenic program but rather induces premature activation of differentiating brown adipocytes through cyclic AMP (cAMP)/protein kinase A (PKA)-mediated lipolysis and fatty acid and glucose oxidation, as well as uncoupling. cAMP/PKA signaling is physiologically activated during neonatal BAT development in wild-type mice and greatly potentiated in mice with genetic deletion of Bscl2 in brown progenitor cells, leading to reduced BAT mass and lipid content during neonatal brown fat formation. However, prolonged overactivation of cAMP/PKA signaling during BAT development ultimately causes apoptosis of brown adipocytes through inflammation, resulting in BAT atrophy and increased overall adiposity in adult mice. These findings reveal a key cellautonomous role for BSCL2 in controlling BAT mass/activity and provide novel insights into therapeutic strategies targeting cAMP/PKA signaling to regulate brown adipocyte function, viability, and metabolic homeostasis.

Original languageEnglish (US)
Pages (from-to)2027-2038
Number of pages12
JournalMolecular and Cellular Biology
Volume36
Issue number15
DOIs
StatePublished - Jan 1 2016

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Congenital Generalized Lipodystrophy
Adipogenesis
Brown Adipose Tissue
Brown Adipocytes
Adenylate Kinase
Cyclic AMP-Dependent Protein Kinases
Cyclic AMP
Homeostasis
White Adipocytes
Lipodystrophy
Lipolysis
Adiposity
Endoplasmic Reticulum
Atrophy
Membrane Proteins
Fatty Acids
Stem Cells
Apoptosis
Inflammation
Lipids

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Berardinelli-Seip congenital lipodystrophy 2/seipin is not required for brown adipogenesis but regulates brown adipose tissue development and function. / Zhou, Hongyi; Black, Stephen Matthew; Benson, Tyler W.; Weintraub, Neal Lee; Chen, Weiqin.

In: Molecular and Cellular Biology, Vol. 36, No. 15, 01.01.2016, p. 2027-2038.

Research output: Contribution to journalArticle

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