Mercaptoethanol treatment of big growth hormone(GH) obtained from human pituitary resulted in a 60% conversion to small GH. Further dissociation was obtained by combined treatment with the reducing agent plus urea. The results indicate that the existence of big GH is dependent upon the formation of inter-polypeptide chain disulfide bonds.
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Clinical Biochemistry
- Biochemistry, medical