Big growth hormone(GH)

Conversion to small gh without peptide bond cleavage

R. Benveniste, M. E. Stachura, M. Szabo, L. A. Frohman

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Mercaptoethanol treatment of big growth hormone(GH) obtained from human pituitary resulted in a 60% conversion to small GH. Further dissociation was obtained by combined treatment with the reducing agent plus urea. The results indicate that the existence of big GH is dependent upon the formation of inter-polypeptide chain disulfide bonds.

Original languageEnglish (US)
Pages (from-to)422-425
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume41
Issue number2
DOIs
StatePublished - Jan 1 1975

Fingerprint

Growth Hormone
Peptides
Human Growth Hormone
Mercaptoethanol
Reducing Agents
Disulfides
Urea

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Big growth hormone(GH) : Conversion to small gh without peptide bond cleavage. / Benveniste, R.; Stachura, M. E.; Szabo, M.; Frohman, L. A.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 41, No. 2, 01.01.1975, p. 422-425.

Research output: Contribution to journalArticle

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